A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair.
The formation of single-stranded breaks in DNA following UV irradiation is assessed in uvrC34 mutants. By altering the SOS DNA-repair system, either by additional mutations or by using drugs affecting transcription or translation, it is shown that such single-stranded breaks require one or more DNA-...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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1988
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| _version_ | 1797058971195932672 |
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| author | Walters, R Wilbraham, H Strike, P Forster, J |
| author_facet | Walters, R Wilbraham, H Strike, P Forster, J |
| author_sort | Walters, R |
| collection | OXFORD |
| description | The formation of single-stranded breaks in DNA following UV irradiation is assessed in uvrC34 mutants. By altering the SOS DNA-repair system, either by additional mutations or by using drugs affecting transcription or translation, it is shown that such single-stranded breaks require one or more DNA-damage-inducible functions. A UV-sensitive strain is characterized as carrying a Tn10 insertion into the uvrC gene. The absence of post-irradiation incision in this strain demonstrates that uvrC function is absolutely required in vivo for the incision stage of excision repair, and suggests that other uvrC mutants are 'leaky'. |
| first_indexed | 2024-03-06T19:57:36Z |
| format | Journal article |
| id | oxford-uuid:262d01e0-19b0-4a5f-b49d-665946533724 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:57:36Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:262d01e0-19b0-4a5f-b49d-6659465337242022-03-26T11:59:27ZA transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:262d01e0-19b0-4a5f-b49d-665946533724EnglishSymplectic Elements at Oxford1988Walters, RWilbraham, HStrike, PForster, JThe formation of single-stranded breaks in DNA following UV irradiation is assessed in uvrC34 mutants. By altering the SOS DNA-repair system, either by additional mutations or by using drugs affecting transcription or translation, it is shown that such single-stranded breaks require one or more DNA-damage-inducible functions. A UV-sensitive strain is characterized as carrying a Tn10 insertion into the uvrC gene. The absence of post-irradiation incision in this strain demonstrates that uvrC function is absolutely required in vivo for the incision stage of excision repair, and suggests that other uvrC mutants are 'leaky'. |
| spellingShingle | Walters, R Wilbraham, H Strike, P Forster, J A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair. |
| title | A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair. |
| title_full | A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair. |
| title_fullStr | A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair. |
| title_full_unstemmed | A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair. |
| title_short | A transposon insertion in the Escherichia coli uvrC gene; UvrC protein is absolutely required for the incision step in excision repair. |
| title_sort | transposon insertion in the escherichia coli uvrc gene uvrc protein is absolutely required for the incision step in excision repair |
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