Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries
To visualise tissues to determine the presence of disease or simply to understand anatomy, it is important to preserve fresh tissue. Fixatives are chemical solutions that preserve tissues to enable microscopic evaluation. However, some fixatives introduce artefact such as shrinkage of cells. Recentl...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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BioScientifica
2021
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| _version_ | 1826310683839430656 |
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| author | Appeltant, R Adeniran, BV Williams, SA |
| author_facet | Appeltant, R Adeniran, BV Williams, SA |
| author_sort | Appeltant, R |
| collection | OXFORD |
| description | To visualise tissues to determine the presence of disease or simply to understand anatomy, it is important to preserve fresh tissue. Fixatives are chemical solutions that preserve tissues to enable microscopic evaluation. However, some fixatives introduce artefact such as shrinkage of cells. Recently, a new fixative, Form-Acetic, was developed that is superior for preserving the structure of ovary tissue and allows investigation of ovary composition. One component of the ovary is hyaluronic acid (HA), which plays a crucial role in normal ovary function and fertility. Importantly, HA is sensitive to different fixative solutions. Therefore, it is meaningful to verify whether Form-Acetic is suitable for detecting HA. In this study, adult mouse ovaries were fixed in Form-Acetic and HA was detected. All HA-containing structures in the ovary were clearly distinguished which proves that the novel fixative allows the detection of HA. |
| first_indexed | 2024-03-07T07:55:34Z |
| format | Journal article |
| id | oxford-uuid:26665cb7-5ded-4b67-ab74-250b604f9d97 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:55:34Z |
| publishDate | 2021 |
| publisher | BioScientifica |
| record_format | dspace |
| spelling | oxford-uuid:26665cb7-5ded-4b67-ab74-250b604f9d972023-08-17T12:13:03ZFixation in Form-Acetic allows hyaluronic acid detection in mouse ovariesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:26665cb7-5ded-4b67-ab74-250b604f9d97EnglishSymplectic ElementsBioScientifica2021Appeltant, RAdeniran, BVWilliams, SATo visualise tissues to determine the presence of disease or simply to understand anatomy, it is important to preserve fresh tissue. Fixatives are chemical solutions that preserve tissues to enable microscopic evaluation. However, some fixatives introduce artefact such as shrinkage of cells. Recently, a new fixative, Form-Acetic, was developed that is superior for preserving the structure of ovary tissue and allows investigation of ovary composition. One component of the ovary is hyaluronic acid (HA), which plays a crucial role in normal ovary function and fertility. Importantly, HA is sensitive to different fixative solutions. Therefore, it is meaningful to verify whether Form-Acetic is suitable for detecting HA. In this study, adult mouse ovaries were fixed in Form-Acetic and HA was detected. All HA-containing structures in the ovary were clearly distinguished which proves that the novel fixative allows the detection of HA. |
| spellingShingle | Appeltant, R Adeniran, BV Williams, SA Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries |
| title | Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries |
| title_full | Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries |
| title_fullStr | Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries |
| title_full_unstemmed | Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries |
| title_short | Fixation in Form-Acetic allows hyaluronic acid detection in mouse ovaries |
| title_sort | fixation in form acetic allows hyaluronic acid detection in mouse ovaries |
| work_keys_str_mv | AT appeltantr fixationinformaceticallowshyaluronicaciddetectioninmouseovaries AT adeniranbv fixationinformaceticallowshyaluronicaciddetectioninmouseovaries AT williamssa fixationinformaceticallowshyaluronicaciddetectioninmouseovaries |