Utilization of keratin-containing biowaste to produce biohydrogen.
A two-stage fermentation system was constructed to test and demonstrate the feasibility of biohydrogen generation from keratin-rich biowaste. We isolated a novel aerobic Bacillus strain (Bacillus licheniformis KK1) that displays outstanding keratinolytic activity. The isolated strain was employed to...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2005
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| _version_ | 1826263901594976256 |
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| author | Bálint, B Bagi, Z Tóth, A Rákhely, G Perei, K Kovács, K |
| author_facet | Bálint, B Bagi, Z Tóth, A Rákhely, G Perei, K Kovács, K |
| author_sort | Bálint, B |
| collection | OXFORD |
| description | A two-stage fermentation system was constructed to test and demonstrate the feasibility of biohydrogen generation from keratin-rich biowaste. We isolated a novel aerobic Bacillus strain (Bacillus licheniformis KK1) that displays outstanding keratinolytic activity. The isolated strain was employed to convert keratin-containing biowaste into a fermentation product that is rich in amino acids and peptides. The process was optimized for the second fermentation step, in which the product of keratin fermentation--supplemented with essential minerals--was metabolized by Thermococcus litoralis, an anaerobic hyperthermophilic archaeon. T. litoralis grew on the keratin hydrolysate and produced hydrogen gas as a physiological fermentation byproduct. Hyperthermophilic cells utilized the keratin hydrolysate in a similar way as their standard nutrient, i.e., bacto-peptone. The generalization of the findings to protein-rich waste treatment and production of biohydrogen is discussed and possible means of further improvements are listed. |
| first_indexed | 2024-03-06T19:59:15Z |
| format | Journal article |
| id | oxford-uuid:26b5a92b-bf7a-4e09-9192-0e866f59c732 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:59:15Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | oxford-uuid:26b5a92b-bf7a-4e09-9192-0e866f59c7322022-03-26T12:02:36ZUtilization of keratin-containing biowaste to produce biohydrogen.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:26b5a92b-bf7a-4e09-9192-0e866f59c732EnglishSymplectic Elements at Oxford2005Bálint, BBagi, ZTóth, ARákhely, GPerei, KKovács, KA two-stage fermentation system was constructed to test and demonstrate the feasibility of biohydrogen generation from keratin-rich biowaste. We isolated a novel aerobic Bacillus strain (Bacillus licheniformis KK1) that displays outstanding keratinolytic activity. The isolated strain was employed to convert keratin-containing biowaste into a fermentation product that is rich in amino acids and peptides. The process was optimized for the second fermentation step, in which the product of keratin fermentation--supplemented with essential minerals--was metabolized by Thermococcus litoralis, an anaerobic hyperthermophilic archaeon. T. litoralis grew on the keratin hydrolysate and produced hydrogen gas as a physiological fermentation byproduct. Hyperthermophilic cells utilized the keratin hydrolysate in a similar way as their standard nutrient, i.e., bacto-peptone. The generalization of the findings to protein-rich waste treatment and production of biohydrogen is discussed and possible means of further improvements are listed. |
| spellingShingle | Bálint, B Bagi, Z Tóth, A Rákhely, G Perei, K Kovács, K Utilization of keratin-containing biowaste to produce biohydrogen. |
| title | Utilization of keratin-containing biowaste to produce biohydrogen. |
| title_full | Utilization of keratin-containing biowaste to produce biohydrogen. |
| title_fullStr | Utilization of keratin-containing biowaste to produce biohydrogen. |
| title_full_unstemmed | Utilization of keratin-containing biowaste to produce biohydrogen. |
| title_short | Utilization of keratin-containing biowaste to produce biohydrogen. |
| title_sort | utilization of keratin containing biowaste to produce biohydrogen |
| work_keys_str_mv | AT balintb utilizationofkeratincontainingbiowastetoproducebiohydrogen AT bagiz utilizationofkeratincontainingbiowastetoproducebiohydrogen AT totha utilizationofkeratincontainingbiowastetoproducebiohydrogen AT rakhelyg utilizationofkeratincontainingbiowastetoproducebiohydrogen AT pereik utilizationofkeratincontainingbiowastetoproducebiohydrogen AT kovacsk utilizationofkeratincontainingbiowastetoproducebiohydrogen |