An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer.
BACKGROUND: Urinary biomarkers are needed to improve the care and reduce the cost of managing bladder cancer. Current biomarkers struggle to identify both high and low-grade cancers due to differing molecular pathways. Changes in microRNA (miR) expression are seen in urothelial carcinogenesis in a...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Nature Publishing Group
2012
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| _version_ | 1797059111972503552 |
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| author | Miah, S Dudziec, E Drayton, R Zlotta, A Morgan, S Rosario, D Hamdy, F Catto, J |
| author_facet | Miah, S Dudziec, E Drayton, R Zlotta, A Morgan, S Rosario, D Hamdy, F Catto, J |
| author_sort | Miah, S |
| collection | OXFORD |
| description | BACKGROUND: Urinary biomarkers are needed to improve the care and reduce the cost of managing bladder cancer. Current biomarkers struggle to identify both high and low-grade cancers due to differing molecular pathways. Changes in microRNA (miR) expression are seen in urothelial carcinogenesis in a phenotype-specific manner. We hypothesised that urinary miRs reflecting low- and high-grade pathways could detect bladder cancers and overcome differences in genetic events seen within the disease. METHODS: We investigated urinary samples (n=121) from patients with bladder cancer (n=68) and age-matched controls (n=53). Fifteen miRs were quantified using real-time PCR. RESULTS: We found that miR is stable within urinary cells despite adverse handling and detected differential expression of 10 miRs from patients with cancer and controls (miRs-15a/15b/24-1/27b/100/135b/203/212/328/1224, ANOVA P<0.05). Individually, miR-1224-3p had the best individual performance with specificity, positive and negative predictive values and concordance of 83%, 83%, 75% and 77%, respectively. The combination of miRs-135b/15b/1224-3p detected bladder cancer with a high sensitivity (94.1%), sufficient specificity (51%) and was correct in 86% of patients (concordance). CONCLUSION: The use of this panel in patients with haematuria would have found 94% of urothelial cell carcinoma, while reducing cystoscopy rates by 26%. However, two invasive cancers (3%) would have been missed. |
| first_indexed | 2024-03-06T19:59:35Z |
| format | Journal article |
| id | oxford-uuid:26ce0752-0a24-44fb-a8d0-8b6ea8ea18a4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T19:59:35Z |
| publishDate | 2012 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:26ce0752-0a24-44fb-a8d0-8b6ea8ea18a42022-03-26T12:03:15ZAn evaluation of urinary microRNA reveals a high sensitivity for bladder cancer.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:26ce0752-0a24-44fb-a8d0-8b6ea8ea18a4EnglishSymplectic Elements at OxfordNature Publishing Group2012Miah, SDudziec, EDrayton, RZlotta, AMorgan, SRosario, DHamdy, FCatto, J BACKGROUND: Urinary biomarkers are needed to improve the care and reduce the cost of managing bladder cancer. Current biomarkers struggle to identify both high and low-grade cancers due to differing molecular pathways. Changes in microRNA (miR) expression are seen in urothelial carcinogenesis in a phenotype-specific manner. We hypothesised that urinary miRs reflecting low- and high-grade pathways could detect bladder cancers and overcome differences in genetic events seen within the disease. METHODS: We investigated urinary samples (n=121) from patients with bladder cancer (n=68) and age-matched controls (n=53). Fifteen miRs were quantified using real-time PCR. RESULTS: We found that miR is stable within urinary cells despite adverse handling and detected differential expression of 10 miRs from patients with cancer and controls (miRs-15a/15b/24-1/27b/100/135b/203/212/328/1224, ANOVA P<0.05). Individually, miR-1224-3p had the best individual performance with specificity, positive and negative predictive values and concordance of 83%, 83%, 75% and 77%, respectively. The combination of miRs-135b/15b/1224-3p detected bladder cancer with a high sensitivity (94.1%), sufficient specificity (51%) and was correct in 86% of patients (concordance). CONCLUSION: The use of this panel in patients with haematuria would have found 94% of urothelial cell carcinoma, while reducing cystoscopy rates by 26%. However, two invasive cancers (3%) would have been missed. |
| spellingShingle | Miah, S Dudziec, E Drayton, R Zlotta, A Morgan, S Rosario, D Hamdy, F Catto, J An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer. |
| title | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer. |
| title_full | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer. |
| title_fullStr | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer. |
| title_full_unstemmed | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer. |
| title_short | An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer. |
| title_sort | evaluation of urinary microrna reveals a high sensitivity for bladder cancer |
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