Gibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor.
DELLA proteins are nuclear repressors of plant gibberellin (GA) responses. Here, we investigate the properties of SLN1, a DELLA protein from barley that is destabilized by GA treatment. Using specific inhibitors of proteasome function, we show that proteasome-mediated protein degradation is necessar...
| Main Authors: | , , , , , , |
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| 格式: | Journal article |
| 語言: | English |
| 出版: |
2002
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| _version_ | 1826263968783532032 |
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| author | Fu, X Richards, D Ait-Ali, T Hynes, L Ougham, H Peng, J Harberd, N |
| author_facet | Fu, X Richards, D Ait-Ali, T Hynes, L Ougham, H Peng, J Harberd, N |
| author_sort | Fu, X |
| collection | OXFORD |
| description | DELLA proteins are nuclear repressors of plant gibberellin (GA) responses. Here, we investigate the properties of SLN1, a DELLA protein from barley that is destabilized by GA treatment. Using specific inhibitors of proteasome function, we show that proteasome-mediated protein degradation is necessary for GA-mediated destabilization of SLN1. We also show that GA responses, such as the aleurone alpha-amylase response and seedling leaf extension growth, require proteasome-dependent GA-mediated SLN1 destabilization. In further experiments with protein kinase and protein phosphatase inhibitors, we identify two additional signaling steps that are necessary for GA response and for GA-mediated destabilization of SLN1. Thus, GA signaling involves protein phosphorylation and dephosphorylation steps and promotes the derepression of GA responses via proteasome-dependent destabilization of DELLA repressors. |
| first_indexed | 2024-03-06T20:00:18Z |
| format | Journal article |
| id | oxford-uuid:2712150f-1fe6-4fd1-ad8f-75fcc6a953ca |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:00:18Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:2712150f-1fe6-4fd1-ad8f-75fcc6a953ca2022-03-26T12:04:38ZGibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2712150f-1fe6-4fd1-ad8f-75fcc6a953caEnglishSymplectic Elements at Oxford2002Fu, XRichards, DAit-Ali, THynes, LOugham, HPeng, JHarberd, NDELLA proteins are nuclear repressors of plant gibberellin (GA) responses. Here, we investigate the properties of SLN1, a DELLA protein from barley that is destabilized by GA treatment. Using specific inhibitors of proteasome function, we show that proteasome-mediated protein degradation is necessary for GA-mediated destabilization of SLN1. We also show that GA responses, such as the aleurone alpha-amylase response and seedling leaf extension growth, require proteasome-dependent GA-mediated SLN1 destabilization. In further experiments with protein kinase and protein phosphatase inhibitors, we identify two additional signaling steps that are necessary for GA response and for GA-mediated destabilization of SLN1. Thus, GA signaling involves protein phosphorylation and dephosphorylation steps and promotes the derepression of GA responses via proteasome-dependent destabilization of DELLA repressors. |
| spellingShingle | Fu, X Richards, D Ait-Ali, T Hynes, L Ougham, H Peng, J Harberd, N Gibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor. |
| title | Gibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor. |
| title_full | Gibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor. |
| title_fullStr | Gibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor. |
| title_full_unstemmed | Gibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor. |
| title_short | Gibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor. |
| title_sort | gibberellin mediated proteasome dependent degradation of the barley della protein sln1 repressor |
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