Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically.
In-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1797059180952027136 |
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| author | Ringrose, J van Solinge, W Mohammed, S O'Flaherty, M van Wijk, R Heck, A Slijper, M |
| author_facet | Ringrose, J van Solinge, W Mohammed, S O'Flaherty, M van Wijk, R Heck, A Slijper, M |
| author_sort | Ringrose, J |
| collection | OXFORD |
| description | In-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of identified proteins. This depletion technique is valuable for proteome studies of human erythrocyte disorders with unknown etiology and of tissue samples that contain blood. |
| first_indexed | 2024-03-06T20:00:33Z |
| format | Journal article |
| id | oxford-uuid:27250514-70cc-42af-b145-24b1d3d02594 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:00:33Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:27250514-70cc-42af-b145-24b1d3d025942022-03-26T12:05:05ZHighly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:27250514-70cc-42af-b145-24b1d3d02594EnglishSymplectic Elements at Oxford2008Ringrose, Jvan Solinge, WMohammed, SO'Flaherty, Mvan Wijk, RHeck, ASlijper, MIn-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of identified proteins. This depletion technique is valuable for proteome studies of human erythrocyte disorders with unknown etiology and of tissue samples that contain blood. |
| spellingShingle | Ringrose, J van Solinge, W Mohammed, S O'Flaherty, M van Wijk, R Heck, A Slijper, M Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically. |
| title | Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically. |
| title_full | Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically. |
| title_fullStr | Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically. |
| title_full_unstemmed | Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically. |
| title_short | Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically. |
| title_sort | highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically |
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