Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber
Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1,2,3,4. Advances in wavefront-shaping methods and computational power have recently allowed for...
| Автори: | , , , , , , , |
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| Формат: | Journal article |
| Опубліковано: |
Springer Nature
2018
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| _version_ | 1826264071379353600 |
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| author | Vasquez-Lopez, S Turcotte, R Koren, V Ploschner, M Padamsey, Z Booth, M Čižmár, T Emptage, N |
| author_facet | Vasquez-Lopez, S Turcotte, R Koren, V Ploschner, M Padamsey, Z Booth, M Čižmár, T Emptage, N |
| author_sort | Vasquez-Lopez, S |
| collection | OXFORD |
| description | Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1,2,3,4. Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)5,6,7. We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo. |
| first_indexed | 2024-03-06T20:01:52Z |
| format | Journal article |
| id | oxford-uuid:2795ae3f-48b3-4f12-a78c-7072d12f8db1 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T20:01:52Z |
| publishDate | 2018 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:2795ae3f-48b3-4f12-a78c-7072d12f8db12022-03-26T12:07:58ZSubcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiberJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2795ae3f-48b3-4f12-a78c-7072d12f8db1Symplectic Elements at OxfordSpringer Nature2018Vasquez-Lopez, STurcotte, RKoren, VPloschner, MPadamsey, ZBooth, MČižmár, TEmptage, NAchieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1,2,3,4. Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)5,6,7. We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo. |
| spellingShingle | Vasquez-Lopez, S Turcotte, R Koren, V Ploschner, M Padamsey, Z Booth, M Čižmár, T Emptage, N Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber |
| title | Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber |
| title_full | Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber |
| title_fullStr | Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber |
| title_full_unstemmed | Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber |
| title_short | Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber |
| title_sort | subcellular spatial resolution achieved for deep brain imaging in vivo using a minimally invasive multimode fiber |
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