Generation and characterization of transgenic mice with the full-length human DMD gene.
We report the generation of mice with an intact and functional copy of the 2.3-megabase human dystrophin gene (hDMD), the largest functional stretch of human DNA thus far integrated into a mouse chromosome. Yeast spheroplasts containing an artificial chromosome with the full-length hDMD gene were fu...
| Main Authors: | , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1826264077430685696 |
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| author | 't Hoen, P de Meijer, E Boer, J Vossen, R Turk, R Maatman, R Davies, K van Ommen, G van Deutekom, J den Dunnen, J |
| author_facet | 't Hoen, P de Meijer, E Boer, J Vossen, R Turk, R Maatman, R Davies, K van Ommen, G van Deutekom, J den Dunnen, J |
| author_sort | 't Hoen, P |
| collection | OXFORD |
| description | We report the generation of mice with an intact and functional copy of the 2.3-megabase human dystrophin gene (hDMD), the largest functional stretch of human DNA thus far integrated into a mouse chromosome. Yeast spheroplasts containing an artificial chromosome with the full-length hDMD gene were fused with mouse embryonic stem cells and were subsequently injected into mouse blastocysts to produce transgenic hDMD mice. Human-specific PCR, Southern blotting, and fluorescent in situ hybridization techniques demonstrated the intactness and stable chromosomal integration of the hDMD gene on mouse chromosome 5. Expression of the transgene was confirmed by RT-PCR and Western blotting. The tissue-specific expression pattern of the different DMD transcripts was maintained. However, the human Dp427p and Dp427m transcripts were expressed at 2-fold higher levels and human Dp427c and Dp260 transcripts were expressed at 2- and 4-fold lower levels than their endogenous counterparts. Ultimate functional proof of the hDMD transgene was obtained by crossing of hDMD mice with dystrophin-deficient mdx mice and dystrophin and utrophin-deficient mdx x Utrn-/- mice. The hDMD transgene rescued the lethal dystrophic phenotype of the mdx x Utrn-/- mice. All signs of muscular dystrophy disappeared in the rescued mice, as demonstrated by histological staining of muscle sections and gene expression profiling experiments. Currently, hDMD mice are extensively used for preclinical testing of sequence-specific therapeutics for the treatment of Duchenne muscular dystrophy. In addition, the hDMD mouse can be used to study the influence of the genomic context on deletion and recombination frequencies, genome stability, and gene expression regulation. |
| first_indexed | 2024-03-06T20:01:58Z |
| format | Journal article |
| id | oxford-uuid:279f179e-eb1f-4ef7-a56d-5b6114de7ae9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:01:58Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:279f179e-eb1f-4ef7-a56d-5b6114de7ae92022-03-26T12:08:01ZGeneration and characterization of transgenic mice with the full-length human DMD gene.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:279f179e-eb1f-4ef7-a56d-5b6114de7ae9EnglishSymplectic Elements at Oxford2008't Hoen, Pde Meijer, EBoer, JVossen, RTurk, RMaatman, RDavies, Kvan Ommen, Gvan Deutekom, Jden Dunnen, JWe report the generation of mice with an intact and functional copy of the 2.3-megabase human dystrophin gene (hDMD), the largest functional stretch of human DNA thus far integrated into a mouse chromosome. Yeast spheroplasts containing an artificial chromosome with the full-length hDMD gene were fused with mouse embryonic stem cells and were subsequently injected into mouse blastocysts to produce transgenic hDMD mice. Human-specific PCR, Southern blotting, and fluorescent in situ hybridization techniques demonstrated the intactness and stable chromosomal integration of the hDMD gene on mouse chromosome 5. Expression of the transgene was confirmed by RT-PCR and Western blotting. The tissue-specific expression pattern of the different DMD transcripts was maintained. However, the human Dp427p and Dp427m transcripts were expressed at 2-fold higher levels and human Dp427c and Dp260 transcripts were expressed at 2- and 4-fold lower levels than their endogenous counterparts. Ultimate functional proof of the hDMD transgene was obtained by crossing of hDMD mice with dystrophin-deficient mdx mice and dystrophin and utrophin-deficient mdx x Utrn-/- mice. The hDMD transgene rescued the lethal dystrophic phenotype of the mdx x Utrn-/- mice. All signs of muscular dystrophy disappeared in the rescued mice, as demonstrated by histological staining of muscle sections and gene expression profiling experiments. Currently, hDMD mice are extensively used for preclinical testing of sequence-specific therapeutics for the treatment of Duchenne muscular dystrophy. In addition, the hDMD mouse can be used to study the influence of the genomic context on deletion and recombination frequencies, genome stability, and gene expression regulation. |
| spellingShingle | 't Hoen, P de Meijer, E Boer, J Vossen, R Turk, R Maatman, R Davies, K van Ommen, G van Deutekom, J den Dunnen, J Generation and characterization of transgenic mice with the full-length human DMD gene. |
| title | Generation and characterization of transgenic mice with the full-length human DMD gene. |
| title_full | Generation and characterization of transgenic mice with the full-length human DMD gene. |
| title_fullStr | Generation and characterization of transgenic mice with the full-length human DMD gene. |
| title_full_unstemmed | Generation and characterization of transgenic mice with the full-length human DMD gene. |
| title_short | Generation and characterization of transgenic mice with the full-length human DMD gene. |
| title_sort | generation and characterization of transgenic mice with the full length human dmd gene |
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