Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos.
We reported previously that the disappearance of cyclin B at the end of mitosis in early Drosophila embryos starts at centrosomes and spreads into the spindle [1]. Here, we used a novel mutation, centrosome fall off (cfo), to investigate whether centrosomes are required to initiate the disappearance...
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| Format: | Journal article |
| Language: | English |
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2000
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| _version_ | 1826264080995844096 |
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| author | Wakefield, J Huang, J Raff, J |
| author_facet | Wakefield, J Huang, J Raff, J |
| author_sort | Wakefield, J |
| collection | OXFORD |
| description | We reported previously that the disappearance of cyclin B at the end of mitosis in early Drosophila embryos starts at centrosomes and spreads into the spindle [1]. Here, we used a novel mutation, centrosome fall off (cfo), to investigate whether centrosomes are required to initiate the disappearance of cyclin B from the spindle. In embryos laid by homozygous cfo mutant mothers, the centrosomes co-ordinately detached from the mitotic spindle during mitosis, and the centrosomeless spindles arrested at anaphase. Cyclin B levels decreased on the detached centrosomes, but not on the arrested centrosomeless spindles, presumably explaining why the spindles arrest in anaphase in these embryos. We found that the expression of a non-degradable cyclin B in embryos also caused an anaphase arrest, but most centrosomes remained attached to the arrested spindles, and non-degradable cyclin B levels remained high on both the centrosomes and spindles. These findings suggest that the disappearance of cyclin B from centrosomes and spindles is closely linked to its destruction, and that a connection between centrosomes and spindles is required for the proper destruction of the spindle-associated cyclin B in early Drosophila embryos. These results may have important implications for the mechanism of the spindle-assembly checkpoint, as they suggest that unattached kinetochores may arrest cells in mitosis, at least in part, by signalling to centrosomes to block the initiation of cyclin B destruction. |
| first_indexed | 2024-03-06T20:02:02Z |
| format | Journal article |
| id | oxford-uuid:27a3f5e6-7228-4a89-9360-326a7ca0f05c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:02:02Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | oxford-uuid:27a3f5e6-7228-4a89-9360-326a7ca0f05c2022-03-26T12:08:07ZCentrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:27a3f5e6-7228-4a89-9360-326a7ca0f05cEnglishSymplectic Elements at Oxford2000Wakefield, JHuang, JRaff, JWe reported previously that the disappearance of cyclin B at the end of mitosis in early Drosophila embryos starts at centrosomes and spreads into the spindle [1]. Here, we used a novel mutation, centrosome fall off (cfo), to investigate whether centrosomes are required to initiate the disappearance of cyclin B from the spindle. In embryos laid by homozygous cfo mutant mothers, the centrosomes co-ordinately detached from the mitotic spindle during mitosis, and the centrosomeless spindles arrested at anaphase. Cyclin B levels decreased on the detached centrosomes, but not on the arrested centrosomeless spindles, presumably explaining why the spindles arrest in anaphase in these embryos. We found that the expression of a non-degradable cyclin B in embryos also caused an anaphase arrest, but most centrosomes remained attached to the arrested spindles, and non-degradable cyclin B levels remained high on both the centrosomes and spindles. These findings suggest that the disappearance of cyclin B from centrosomes and spindles is closely linked to its destruction, and that a connection between centrosomes and spindles is required for the proper destruction of the spindle-associated cyclin B in early Drosophila embryos. These results may have important implications for the mechanism of the spindle-assembly checkpoint, as they suggest that unattached kinetochores may arrest cells in mitosis, at least in part, by signalling to centrosomes to block the initiation of cyclin B destruction. |
| spellingShingle | Wakefield, J Huang, J Raff, J Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos. |
| title | Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos. |
| title_full | Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos. |
| title_fullStr | Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos. |
| title_full_unstemmed | Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos. |
| title_short | Centrosomes have a role in regulating the destruction of cyclin B in early Drosophila embryos. |
| title_sort | centrosomes have a role in regulating the destruction of cyclin b in early drosophila embryos |
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