Augmentation of cell-mediated responses in vitro by a monoclonal anti-helper factor antibody.
Previous studies have shown that monoclonal antibody AF3.44.4 has specificity for a constant region determinant on mouse antigen-specific helper factors and that it also binds to cultured T cells with functional helper cell characteristics. The antibody synergizes with antigen to enhance in vitro an...
| Main Authors: | , , , , |
|---|---|
| 格式: | Journal article |
| 語言: | English |
| 出版: |
1985
|
| _version_ | 1826264187827912704 |
|---|---|
| author | James, R Sunshine, G Edwards, S Maudsley, D Feldmann, M |
| author_facet | James, R Sunshine, G Edwards, S Maudsley, D Feldmann, M |
| author_sort | James, R |
| collection | OXFORD |
| description | Previous studies have shown that monoclonal antibody AF3.44.4 has specificity for a constant region determinant on mouse antigen-specific helper factors and that it also binds to cultured T cells with functional helper cell characteristics. The antibody synergizes with antigen to enhance in vitro antibody responses; here we demonstrate that it will also enhance cell-mediated responses in vitro such as in the generation of proliferating cells in mixed lymphocyte responses and in the generation of specific killer cells in cytotoxic T lymphocyte cultures. The mechanism of AF3.44.4-generated enhancement was investigated. Increased levels of the lymphokines IL-2 and BCDF were detected in supernatants of AF3.44.4-treated cultures but the antibody itself could not replace interleukin-2 (IL-2), and would not stimulate primed cells in the absence of antigen. This type of monoclonal antibody which augments immunological responses in an antigen-dependent fashion may provide a new class of immunostimulant and a new approach to augmenting the responses of weak immunogens. |
| first_indexed | 2024-03-06T20:03:43Z |
| format | Journal article |
| id | oxford-uuid:283728a5-28f8-4af5-93f0-a9cd1710d346 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:03:43Z |
| publishDate | 1985 |
| record_format | dspace |
| spelling | oxford-uuid:283728a5-28f8-4af5-93f0-a9cd1710d3462022-03-26T12:11:29ZAugmentation of cell-mediated responses in vitro by a monoclonal anti-helper factor antibody.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:283728a5-28f8-4af5-93f0-a9cd1710d346EnglishSymplectic Elements at Oxford1985James, RSunshine, GEdwards, SMaudsley, DFeldmann, MPrevious studies have shown that monoclonal antibody AF3.44.4 has specificity for a constant region determinant on mouse antigen-specific helper factors and that it also binds to cultured T cells with functional helper cell characteristics. The antibody synergizes with antigen to enhance in vitro antibody responses; here we demonstrate that it will also enhance cell-mediated responses in vitro such as in the generation of proliferating cells in mixed lymphocyte responses and in the generation of specific killer cells in cytotoxic T lymphocyte cultures. The mechanism of AF3.44.4-generated enhancement was investigated. Increased levels of the lymphokines IL-2 and BCDF were detected in supernatants of AF3.44.4-treated cultures but the antibody itself could not replace interleukin-2 (IL-2), and would not stimulate primed cells in the absence of antigen. This type of monoclonal antibody which augments immunological responses in an antigen-dependent fashion may provide a new class of immunostimulant and a new approach to augmenting the responses of weak immunogens. |
| spellingShingle | James, R Sunshine, G Edwards, S Maudsley, D Feldmann, M Augmentation of cell-mediated responses in vitro by a monoclonal anti-helper factor antibody. |
| title | Augmentation of cell-mediated responses in vitro by a monoclonal anti-helper factor antibody. |
| title_full | Augmentation of cell-mediated responses in vitro by a monoclonal anti-helper factor antibody. |
| title_fullStr | Augmentation of cell-mediated responses in vitro by a monoclonal anti-helper factor antibody. |
| title_full_unstemmed | Augmentation of cell-mediated responses in vitro by a monoclonal anti-helper factor antibody. |
| title_short | Augmentation of cell-mediated responses in vitro by a monoclonal anti-helper factor antibody. |
| title_sort | augmentation of cell mediated responses in vitro by a monoclonal anti helper factor antibody |
| work_keys_str_mv | AT jamesr augmentationofcellmediatedresponsesinvitrobyamonoclonalantihelperfactorantibody AT sunshineg augmentationofcellmediatedresponsesinvitrobyamonoclonalantihelperfactorantibody AT edwardss augmentationofcellmediatedresponsesinvitrobyamonoclonalantihelperfactorantibody AT maudsleyd augmentationofcellmediatedresponsesinvitrobyamonoclonalantihelperfactorantibody AT feldmannm augmentationofcellmediatedresponsesinvitrobyamonoclonalantihelperfactorantibody |