Gene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10.
One hundred progeny from each of two intersubspecific mouse backcrosses were used to construct a comparative genetic map of a region of mouse chromosome 10 (MMU10) that is homologous to the distal tip of the long arm of human chromosome 21 (HSA21). The analysis included five genes and three simple s...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1994
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| _version_ | 1797059430843416576 |
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| author | Irving, N Cabin, D Swanson, D Reeves, R |
| author_facet | Irving, N Cabin, D Swanson, D Reeves, R |
| author_sort | Irving, N |
| collection | OXFORD |
| description | One hundred progeny from each of two intersubspecific mouse backcrosses were used to construct a comparative genetic map of a region of mouse chromosome 10 (MMU10) that is homologous to the distal tip of the long arm of human chromosome 21 (HSA21). The analysis included five genes and three simple sequence repeat markers, two of which flanked the HSA21-homologous cluster on either side. Analysis of 200 backcross progeny detected at least one crossover between each pair of adjacent genes and demonstrated that the proximal to distal orientation of the cluster was reversed between human and mouse. The order was determined to be Fyn-1-D10Mit20-S100b-Col6a1-Itgb2-Pfkl+ ++/D10Mit7-D10Mit11. Comparative mapping supports the order of corresponding markers on HSA21 determined using pulsed-field gel electrophoresis and radiation hybrid line data. However, sequence tagged site content mapping of human yeast artificial chromosomes (YACs) yielded conflicting data on the relative positions of human COL6A1 and S100B on HSA21. This discrepancy was resolved here by demonstrating that several key YACs used in the human contig analysis were mistyped for S100B. The murine map reported here provides a scaffold for construction of physical maps and yeast artificial chromosome contigs that will be useful in the development of mouse models for the study of Down syndrome. |
| first_indexed | 2024-03-06T20:04:09Z |
| format | Journal article |
| id | oxford-uuid:28578b17-9174-45c9-9f60-4746e2d9c33d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:04:09Z |
| publishDate | 1994 |
| record_format | dspace |
| spelling | oxford-uuid:28578b17-9174-45c9-9f60-4746e2d9c33d2022-03-26T12:12:24ZGene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:28578b17-9174-45c9-9f60-4746e2d9c33dEnglishSymplectic Elements at Oxford1994Irving, NCabin, DSwanson, DReeves, ROne hundred progeny from each of two intersubspecific mouse backcrosses were used to construct a comparative genetic map of a region of mouse chromosome 10 (MMU10) that is homologous to the distal tip of the long arm of human chromosome 21 (HSA21). The analysis included five genes and three simple sequence repeat markers, two of which flanked the HSA21-homologous cluster on either side. Analysis of 200 backcross progeny detected at least one crossover between each pair of adjacent genes and demonstrated that the proximal to distal orientation of the cluster was reversed between human and mouse. The order was determined to be Fyn-1-D10Mit20-S100b-Col6a1-Itgb2-Pfkl+ ++/D10Mit7-D10Mit11. Comparative mapping supports the order of corresponding markers on HSA21 determined using pulsed-field gel electrophoresis and radiation hybrid line data. However, sequence tagged site content mapping of human yeast artificial chromosomes (YACs) yielded conflicting data on the relative positions of human COL6A1 and S100B on HSA21. This discrepancy was resolved here by demonstrating that several key YACs used in the human contig analysis were mistyped for S100B. The murine map reported here provides a scaffold for construction of physical maps and yeast artificial chromosome contigs that will be useful in the development of mouse models for the study of Down syndrome. |
| spellingShingle | Irving, N Cabin, D Swanson, D Reeves, R Gene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10. |
| title | Gene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10. |
| title_full | Gene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10. |
| title_fullStr | Gene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10. |
| title_full_unstemmed | Gene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10. |
| title_short | Gene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10. |
| title_sort | gene order is conserved within the human chromosome 21 linkage group on mouse chromosome 10 |
| work_keys_str_mv | AT irvingn geneorderisconservedwithinthehumanchromosome21linkagegrouponmousechromosome10 AT cabind geneorderisconservedwithinthehumanchromosome21linkagegrouponmousechromosome10 AT swansond geneorderisconservedwithinthehumanchromosome21linkagegrouponmousechromosome10 AT reevesr geneorderisconservedwithinthehumanchromosome21linkagegrouponmousechromosome10 |