Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy
Nanoparticles are of increasing importance in biomedicine but quantification is problematic because current methods depend on indirect measurements at low resolution. Here we describe a new high-resolution method for measuring and quantifying nanoparticles in suspension. It involves premixing nanopa...
| Hlavní autoři: | , , , , , , , , , |
|---|---|
| Médium: | Journal article |
| Vydáno: |
Nature Publishing Group
2016
|
| _version_ | 1826264233920167936 |
|---|---|
| author | Hacker, C Asadi, J Pliotas, C Ferguson, S Sherry, L Marius, P Tello, J Jackson, D Naismith, J Lucocq, J |
| author_facet | Hacker, C Asadi, J Pliotas, C Ferguson, S Sherry, L Marius, P Tello, J Jackson, D Naismith, J Lucocq, J |
| author_sort | Hacker, C |
| collection | OXFORD |
| description | Nanoparticles are of increasing importance in biomedicine but quantification is problematic because current methods depend on indirect measurements at low resolution. Here we describe a new high-resolution method for measuring and quantifying nanoparticles in suspension. It involves premixing nanoparticles in a hydrophilic support medium (methylcellulose) before introducing heavy metal stains for visualization in small air-dried droplets by transmission electron microscopy (TEM). The use of methylcellulose avoids artifacts of conventional negative stain-TEM by (1) restricting interactions between the nanoparticles, (2) inhibiting binding to the specimen support films and (3) reducing compression after drying. Methylcellulose embedment provides effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualization of nanoparticle populations in droplets of known size. These qualities facilitate unbiased sampling, rapid size measurement and estimation of nanoparticle numbers by means of ratio counting using a colloidal gold calibrant. Specimen preparation and quantification take minutes and require a few microliters of sample using only basic laboratory equipment and a standard TEM. |
| first_indexed | 2024-03-06T20:04:30Z |
| format | Journal article |
| id | oxford-uuid:287aeed3-e915-471e-b89c-dbd3fa5a306f |
| institution | University of Oxford |
| last_indexed | 2024-03-06T20:04:30Z |
| publishDate | 2016 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:287aeed3-e915-471e-b89c-dbd3fa5a306f2022-03-26T12:13:03ZNanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopyJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:287aeed3-e915-471e-b89c-dbd3fa5a306fSymplectic Elements at OxfordNature Publishing Group2016Hacker, CAsadi, JPliotas, CFerguson, SSherry, LMarius, PTello, JJackson, DNaismith, JLucocq, JNanoparticles are of increasing importance in biomedicine but quantification is problematic because current methods depend on indirect measurements at low resolution. Here we describe a new high-resolution method for measuring and quantifying nanoparticles in suspension. It involves premixing nanoparticles in a hydrophilic support medium (methylcellulose) before introducing heavy metal stains for visualization in small air-dried droplets by transmission electron microscopy (TEM). The use of methylcellulose avoids artifacts of conventional negative stain-TEM by (1) restricting interactions between the nanoparticles, (2) inhibiting binding to the specimen support films and (3) reducing compression after drying. Methylcellulose embedment provides effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualization of nanoparticle populations in droplets of known size. These qualities facilitate unbiased sampling, rapid size measurement and estimation of nanoparticle numbers by means of ratio counting using a colloidal gold calibrant. Specimen preparation and quantification take minutes and require a few microliters of sample using only basic laboratory equipment and a standard TEM. |
| spellingShingle | Hacker, C Asadi, J Pliotas, C Ferguson, S Sherry, L Marius, P Tello, J Jackson, D Naismith, J Lucocq, J Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy |
| title | Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy |
| title_full | Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy |
| title_fullStr | Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy |
| title_full_unstemmed | Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy |
| title_short | Nanoparticle suspensions enclosed in methylcellulose: a new approach for quantifying nanoparticles in transmission electron microscopy |
| title_sort | nanoparticle suspensions enclosed in methylcellulose a new approach for quantifying nanoparticles in transmission electron microscopy |
| work_keys_str_mv | AT hackerc nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT asadij nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT pliotasc nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT fergusons nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT sherryl nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT mariusp nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT telloj nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT jacksond nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT naismithj nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy AT lucocqj nanoparticlesuspensionsenclosedinmethylcelluloseanewapproachforquantifyingnanoparticlesintransmissionelectronmicroscopy |