Medium-throughput production of recombinant human proteins: protein production in insect cells.
This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tac...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2014
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| _version_ | 1797059492797480960 |
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| author | Mahajan, P Strain-Damerell, C Gileadi, O Burgess-Brown, N |
| author_facet | Mahajan, P Strain-Damerell, C Gileadi, O Burgess-Brown, N |
| author_sort | Mahajan, P |
| collection | OXFORD |
| description | This chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA-DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cell culture and describe the procedures involved in producing protein from liter-scale cultures. Although not discussed in this chapter, the same process can also be applied to integral membrane proteins (IMPs) with slight adaptations to the purification procedure. |
| first_indexed | 2024-03-06T20:05:02Z |
| format | Journal article |
| id | oxford-uuid:28a01faf-5a27-439a-844c-dc3657b3b1a0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:05:02Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:28a01faf-5a27-439a-844c-dc3657b3b1a02022-03-26T12:14:03ZMedium-throughput production of recombinant human proteins: protein production in insect cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:28a01faf-5a27-439a-844c-dc3657b3b1a0EnglishSymplectic Elements at Oxford2014Mahajan, PStrain-Damerell, CGileadi, OBurgess-Brown, NThis chapter describes the step-by-step methods employed by the Structural Genomics Consortium (SGC) for screening and producing proteins in the baculovirus expression vector system (BEVS). This eukaryotic expression system was selected and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA-DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cell culture and describe the procedures involved in producing protein from liter-scale cultures. Although not discussed in this chapter, the same process can also be applied to integral membrane proteins (IMPs) with slight adaptations to the purification procedure. |
| spellingShingle | Mahajan, P Strain-Damerell, C Gileadi, O Burgess-Brown, N Medium-throughput production of recombinant human proteins: protein production in insect cells. |
| title | Medium-throughput production of recombinant human proteins: protein production in insect cells. |
| title_full | Medium-throughput production of recombinant human proteins: protein production in insect cells. |
| title_fullStr | Medium-throughput production of recombinant human proteins: protein production in insect cells. |
| title_full_unstemmed | Medium-throughput production of recombinant human proteins: protein production in insect cells. |
| title_short | Medium-throughput production of recombinant human proteins: protein production in insect cells. |
| title_sort | medium throughput production of recombinant human proteins protein production in insect cells |
| work_keys_str_mv | AT mahajanp mediumthroughputproductionofrecombinanthumanproteinsproteinproductionininsectcells AT straindamerellc mediumthroughputproductionofrecombinanthumanproteinsproteinproductionininsectcells AT gileadio mediumthroughputproductionofrecombinanthumanproteinsproteinproductionininsectcells AT burgessbrownn mediumthroughputproductionofrecombinanthumanproteinsproteinproductionininsectcells |