Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors.
Invariant NKT (iNKT) cells are a subset of highly conserved immunoregulatory T cells that modify a variety of immune responses, including alloreactivity. Central to their function is the interaction of the invariant TCR with glycosphingolipid (GSL) ligands presented by the nonpolymorphic MHC class I...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2008
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| _version_ | 1797059719419920384 |
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| author | Patterson, S Chaidos, A Neville, D Poggi, A Butters, T Roberts, I Karadimitris, A |
| author_facet | Patterson, S Chaidos, A Neville, D Poggi, A Butters, T Roberts, I Karadimitris, A |
| author_sort | Patterson, S |
| collection | OXFORD |
| description | Invariant NKT (iNKT) cells are a subset of highly conserved immunoregulatory T cells that modify a variety of immune responses, including alloreactivity. Central to their function is the interaction of the invariant TCR with glycosphingolipid (GSL) ligands presented by the nonpolymorphic MHC class I molecule CD1d and their ability to secrete rapidly large amounts of immunomodulatory cytokines when activated. Whether iNKT cells, like NK and conventional T cells, can directly display alloreactivity is not known. We show in this study that human iNKT cells and APC can establish a direct cross-talk leading to preferential maturation of allogeneic APC and a considerably higher reactivity of iNKT cells cultured with allogeneic rather that autologous APC. Although the allogeneic activation of iNKT cells is invariant TCR-CD1d interaction-dependent, GSL profiling suggests it does not involve the recognition of disparate CD1d/GSL complexes. Instead, we show that contrary to previous reports, iNKT cells, like NK and T cells, express killer Ig receptors at a frequency similar to that of conventional T cells and that iNKT cell allogeneic activation requires up-regulation and function of activating killer Ig receptors. Thus, iNKT cells can display alloreactivity, for which they use mechanisms characteristic of both NK and conventional T cells. |
| first_indexed | 2024-03-06T20:08:13Z |
| format | Journal article |
| id | oxford-uuid:29a29b92-3221-4a85-92a9-60f742c6c4a7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:08:13Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:29a29b92-3221-4a85-92a9-60f742c6c4a72022-03-26T12:20:19ZHuman invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:29a29b92-3221-4a85-92a9-60f742c6c4a7EnglishSymplectic Elements at Oxford2008Patterson, SChaidos, ANeville, DPoggi, AButters, TRoberts, IKaradimitris, AInvariant NKT (iNKT) cells are a subset of highly conserved immunoregulatory T cells that modify a variety of immune responses, including alloreactivity. Central to their function is the interaction of the invariant TCR with glycosphingolipid (GSL) ligands presented by the nonpolymorphic MHC class I molecule CD1d and their ability to secrete rapidly large amounts of immunomodulatory cytokines when activated. Whether iNKT cells, like NK and conventional T cells, can directly display alloreactivity is not known. We show in this study that human iNKT cells and APC can establish a direct cross-talk leading to preferential maturation of allogeneic APC and a considerably higher reactivity of iNKT cells cultured with allogeneic rather that autologous APC. Although the allogeneic activation of iNKT cells is invariant TCR-CD1d interaction-dependent, GSL profiling suggests it does not involve the recognition of disparate CD1d/GSL complexes. Instead, we show that contrary to previous reports, iNKT cells, like NK and T cells, express killer Ig receptors at a frequency similar to that of conventional T cells and that iNKT cell allogeneic activation requires up-regulation and function of activating killer Ig receptors. Thus, iNKT cells can display alloreactivity, for which they use mechanisms characteristic of both NK and conventional T cells. |
| spellingShingle | Patterson, S Chaidos, A Neville, D Poggi, A Butters, T Roberts, I Karadimitris, A Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors. |
| title | Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors. |
| title_full | Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors. |
| title_fullStr | Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors. |
| title_full_unstemmed | Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors. |
| title_short | Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors. |
| title_sort | human invariant nkt cells display alloreactivity instructed by invariant tcr cd1d interaction and killer ig receptors |
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