Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model.
BACKGROUND: Antibodies play a major role in the protection against influenza virus in human. However, the antibody level is usually short-lived and the cellular mechanisms underlying influenza virus-specific antibody response to acute infection remain unclear. METHODS: We studied the kinetics and...
| Main Authors: | , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
University of Chicago Press
2014
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| _version_ | 1797059721571598336 |
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| author | Huang, K Li, C Clutterbuck, E Chui, C Wilkinson, T Gilbert, A Oxford, J Lambkin-Williams, R Lin, T Mcmichael, A Xu, X |
| author_facet | Huang, K Li, C Clutterbuck, E Chui, C Wilkinson, T Gilbert, A Oxford, J Lambkin-Williams, R Lin, T Mcmichael, A Xu, X |
| author_sort | Huang, K |
| collection | OXFORD |
| description | BACKGROUND: Antibodies play a major role in the protection against influenza virus in human. However, the antibody level is usually short-lived and the cellular mechanisms underlying influenza virus-specific antibody response to acute infection remain unclear. METHODS: We studied the kinetics and magnitude of influenza virus-specific B-cell and serum antibody responses in relation to virus replication during the course of influenza infection in healthy adult volunteers who were previously seronegative and experimentally infected with seasonal influenza H1N1 A/Brisbane/59/07 virus. RESULTS: Our data demonstrated a robust expansion of the virus-specific antibody-secreting cells (ASCs) and memory B cells in the peripheral blood, which correlated with both the throat viral load and the duration of viral shedding. The ASC response was obviously detected on day 7 post-infection when the virus was completely cleared from nasal samples, and serum hemagglutination-inhibition antibodies were still undetectable. On day 28 postinfection, influenza virus-specific B cells were further identified from the circulating compartment of isotype-switched B cells. CONCLUSIONS: Virus-specific ASCs could be the earliest marker of B-cell response to a new flu virus infection, such as H7N9 in humans. |
| first_indexed | 2024-03-06T20:08:14Z |
| format | Journal article |
| id | oxford-uuid:29a5770d-55e3-4155-bd72-10d2239d0ec0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:08:14Z |
| publishDate | 2014 |
| publisher | University of Chicago Press |
| record_format | dspace |
| spelling | oxford-uuid:29a5770d-55e3-4155-bd72-10d2239d0ec02022-03-26T12:20:29ZVirus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:29a5770d-55e3-4155-bd72-10d2239d0ec0EnglishSymplectic Elements at OxfordUniversity of Chicago Press2014Huang, KLi, CClutterbuck, EChui, CWilkinson, TGilbert, AOxford, JLambkin-Williams, RLin, TMcmichael, AXu, XBACKGROUND: Antibodies play a major role in the protection against influenza virus in human. However, the antibody level is usually short-lived and the cellular mechanisms underlying influenza virus-specific antibody response to acute infection remain unclear. METHODS: We studied the kinetics and magnitude of influenza virus-specific B-cell and serum antibody responses in relation to virus replication during the course of influenza infection in healthy adult volunteers who were previously seronegative and experimentally infected with seasonal influenza H1N1 A/Brisbane/59/07 virus. RESULTS: Our data demonstrated a robust expansion of the virus-specific antibody-secreting cells (ASCs) and memory B cells in the peripheral blood, which correlated with both the throat viral load and the duration of viral shedding. The ASC response was obviously detected on day 7 post-infection when the virus was completely cleared from nasal samples, and serum hemagglutination-inhibition antibodies were still undetectable. On day 28 postinfection, influenza virus-specific B cells were further identified from the circulating compartment of isotype-switched B cells. CONCLUSIONS: Virus-specific ASCs could be the earliest marker of B-cell response to a new flu virus infection, such as H7N9 in humans. |
| spellingShingle | Huang, K Li, C Clutterbuck, E Chui, C Wilkinson, T Gilbert, A Oxford, J Lambkin-Williams, R Lin, T Mcmichael, A Xu, X Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. |
| title | Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. |
| title_full | Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. |
| title_fullStr | Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. |
| title_full_unstemmed | Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. |
| title_short | Virus-specific antibody secreting cell, memory B-cell, and sero-antibody responses in the human influenza challenge model. |
| title_sort | virus specific antibody secreting cell memory b cell and sero antibody responses in the human influenza challenge model |
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