Formation of germinal centres in the rat
<p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">This thesis demonstrates a method based on the monoclonal antibody (mAb) HIS22 for distinguishing germinal centre B cells (GCC-B) of rat lymph nodes both in situ and in suspension. In two-colour flow cytometric studies, GCC-B...
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| Format: | Thesis |
| Language: | English |
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1988
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| _version_ | 1817932384610287616 |
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| author | Vonderheide, R |
| author2 | Hunt, S |
| author_facet | Hunt, S Vonderheide, R |
| author_sort | Vonderheide, R |
| collection | OXFORD |
| description | <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">This thesis demonstrates a method based on the monoclonal antibody (mAb) HIS22 for distinguishing germinal centre B cells (GCC-B) of rat lymph nodes both in situ and in suspension. In two-colour flow cytometric studies, GCC-B were clearly identified as HIS22-negative, Ig-kappa-positive. For non-irradiated rats injected intravenously with B cells of the opposite kappa allotype, the relative distribution of donor B cells in GCs seven days after immunisation was calculated from FACS analysis using HIS22 and two anti-kappa alloantibodies. There were two general findings using this assay: (1) IgD-negative B cells found in the thoracic duct preferentially act as germinal centre precursor cells (GCPC), especially IgD-negative (memory) B cells isolated from primed donors, and (2) B cells primed to the specific, immunising antigen can immigrate into developing or well- developed germinal centres while unprimed B cells, one day or more after immunisation, cannot.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">A subset of T-helper cells, as indicated by their labelling with mAb ER3, make up the vast majority of T cells found in lymph node or splenic germinal centres; a necessary role for these cells in helping B cells form germinal centres was found. A subset of ER3-positive T-helper cells is 0X22-negative, implying that some ER3-positive cells are memory T cells.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">A previous suggestion that the Oxford mAb 157.6 recognises rat IgD was confirmed by immunoprecipitating the antigen from cell-surface iodinated proteins and comparing the pattern with that of mAb MARD-3 (anti-6 heavy chain). 157.6 labels 40%-60% of all IgD-positive cells (depending on the strain) and therefore identifies a serological heterogeneity of rat IgD.</p> |
| first_indexed | 2024-03-06T20:10:18Z |
| format | Thesis |
| id | oxford-uuid:2a533d75-468a-44b0-a07c-60c6d8f6b17a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-12-09T03:37:04Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:2a533d75-468a-44b0-a07c-60c6d8f6b17a2024-12-01T20:21:16ZFormation of germinal centres in the ratThesishttp://purl.org/coar/resource_type/c_db06uuid:2a533d75-468a-44b0-a07c-60c6d8f6b17aMonoclonal antibodiesGerminal centersB cellsT cellsEnglishPolonsky Theses Digitisation Project1988Vonderheide, RHunt, S<p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">This thesis demonstrates a method based on the monoclonal antibody (mAb) HIS22 for distinguishing germinal centre B cells (GCC-B) of rat lymph nodes both in situ and in suspension. In two-colour flow cytometric studies, GCC-B were clearly identified as HIS22-negative, Ig-kappa-positive. For non-irradiated rats injected intravenously with B cells of the opposite kappa allotype, the relative distribution of donor B cells in GCs seven days after immunisation was calculated from FACS analysis using HIS22 and two anti-kappa alloantibodies. There were two general findings using this assay: (1) IgD-negative B cells found in the thoracic duct preferentially act as germinal centre precursor cells (GCPC), especially IgD-negative (memory) B cells isolated from primed donors, and (2) B cells primed to the specific, immunising antigen can immigrate into developing or well- developed germinal centres while unprimed B cells, one day or more after immunisation, cannot.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">A subset of T-helper cells, as indicated by their labelling with mAb ER3, make up the vast majority of T cells found in lymph node or splenic germinal centres; a necessary role for these cells in helping B cells form germinal centres was found. A subset of ER3-positive T-helper cells is 0X22-negative, implying that some ER3-positive cells are memory T cells.</p> <p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">A previous suggestion that the Oxford mAb 157.6 recognises rat IgD was confirmed by immunoprecipitating the antigen from cell-surface iodinated proteins and comparing the pattern with that of mAb MARD-3 (anti-6 heavy chain). 157.6 labels 40%-60% of all IgD-positive cells (depending on the strain) and therefore identifies a serological heterogeneity of rat IgD.</p> |
| spellingShingle | Monoclonal antibodies Germinal centers B cells T cells Vonderheide, R Formation of germinal centres in the rat |
| title | Formation of germinal centres in the rat |
| title_full | Formation of germinal centres in the rat |
| title_fullStr | Formation of germinal centres in the rat |
| title_full_unstemmed | Formation of germinal centres in the rat |
| title_short | Formation of germinal centres in the rat |
| title_sort | formation of germinal centres in the rat |
| topic | Monoclonal antibodies Germinal centers B cells T cells |
| work_keys_str_mv | AT vonderheider formationofgerminalcentresintherat |