Decoupled and simultaneous three-dimensional imaging and optical manipulation through a single objective
The combination of optical manipulation and threedimensional imaging is a central technique in fields ranging from medicine to physics. Using the objective lens simultaneously for optical trapping and imaging, however, inherently confines the trapping and imaging planes to the same focal plane. Here...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Published: |
Optical Society of America
2014
|
| _version_ | 1797059979219304448 |
|---|---|
| author | Curran, A Tuohy, S Aarts, D Booth, M Wilson, T Dullens, R |
| author_facet | Curran, A Tuohy, S Aarts, D Booth, M Wilson, T Dullens, R |
| author_sort | Curran, A |
| collection | OXFORD |
| description | The combination of optical manipulation and threedimensional imaging is a central technique in fields ranging from medicine to physics. Using the objective lens simultaneously for optical trapping and imaging, however, inherently confines the trapping and imaging planes to the same focal plane. Here, we combine remote refocusing microscopy and optical trapping to optically decouple the imaging and trapping planes, achieving aberration-free three-dimensional imaging and simultaneous, decoupled optical trapping without the need for feedback or aberration corrections. We demonstrate our approach by directly imaging the flow field around optically trapped spheres in three dimensions. Due to its compatibility with other imaging and optical manipulation techniques, our approach is relevant to the wide range of fields that combine imaging and optical manipulation, such as physical chemistry, cell biology, and soft matter. |
| first_indexed | 2024-03-06T20:11:17Z |
| format | Journal article |
| id | oxford-uuid:2aa33cec-f246-4f84-b85c-efebb3147f47 |
| institution | University of Oxford |
| last_indexed | 2024-03-06T20:11:17Z |
| publishDate | 2014 |
| publisher | Optical Society of America |
| record_format | dspace |
| spelling | oxford-uuid:2aa33cec-f246-4f84-b85c-efebb3147f472022-03-26T12:26:17ZDecoupled and simultaneous three-dimensional imaging and optical manipulation through a single objectiveJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2aa33cec-f246-4f84-b85c-efebb3147f47Symplectic Elements at OxfordOptical Society of America2014Curran, ATuohy, SAarts, DBooth, MWilson, TDullens, RThe combination of optical manipulation and threedimensional imaging is a central technique in fields ranging from medicine to physics. Using the objective lens simultaneously for optical trapping and imaging, however, inherently confines the trapping and imaging planes to the same focal plane. Here, we combine remote refocusing microscopy and optical trapping to optically decouple the imaging and trapping planes, achieving aberration-free three-dimensional imaging and simultaneous, decoupled optical trapping without the need for feedback or aberration corrections. We demonstrate our approach by directly imaging the flow field around optically trapped spheres in three dimensions. Due to its compatibility with other imaging and optical manipulation techniques, our approach is relevant to the wide range of fields that combine imaging and optical manipulation, such as physical chemistry, cell biology, and soft matter. |
| spellingShingle | Curran, A Tuohy, S Aarts, D Booth, M Wilson, T Dullens, R Decoupled and simultaneous three-dimensional imaging and optical manipulation through a single objective |
| title | Decoupled and simultaneous three-dimensional imaging and optical manipulation through a single objective |
| title_full | Decoupled and simultaneous three-dimensional imaging and optical manipulation through a single objective |
| title_fullStr | Decoupled and simultaneous three-dimensional imaging and optical manipulation through a single objective |
| title_full_unstemmed | Decoupled and simultaneous three-dimensional imaging and optical manipulation through a single objective |
| title_short | Decoupled and simultaneous three-dimensional imaging and optical manipulation through a single objective |
| title_sort | decoupled and simultaneous three dimensional imaging and optical manipulation through a single objective |
| work_keys_str_mv | AT currana decoupledandsimultaneousthreedimensionalimagingandopticalmanipulationthroughasingleobjective AT tuohys decoupledandsimultaneousthreedimensionalimagingandopticalmanipulationthroughasingleobjective AT aartsd decoupledandsimultaneousthreedimensionalimagingandopticalmanipulationthroughasingleobjective AT boothm decoupledandsimultaneousthreedimensionalimagingandopticalmanipulationthroughasingleobjective AT wilsont decoupledandsimultaneousthreedimensionalimagingandopticalmanipulationthroughasingleobjective AT dullensr decoupledandsimultaneousthreedimensionalimagingandopticalmanipulationthroughasingleobjective |