Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions.
We describe a general mass spectrometry approach to determine subunit stoichiometry and lipid binding in intact membrane protein complexes. By exploring conditions for preserving interactions during transmission into the gas phase and for optimally stripping away detergent, by subjecting the complex...
Main Authors: | Barrera, N, Isaacson, S, Zhou, M, Bavro, V, Welch, A, Schaedler, T, Seeger, M, Miguel, R, Korkhov, V, van Veen, H, Venter, H, Walmsley, A, Tate, C, Robinson, C |
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Format: | Journal article |
Language: | English |
Published: |
2009
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