Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay.
Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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| _version_ | 1797060024944558080 |
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| author | Chantratita, N Tandhavanant, S Wongsuvan, G Wuthiekanun, V Teerawattanasook, N Day, N Limmathurotsakul, D Peacock, S |
| author_facet | Chantratita, N Tandhavanant, S Wongsuvan, G Wuthiekanun, V Teerawattanasook, N Day, N Limmathurotsakul, D Peacock, S |
| author_sort | Chantratita, N |
| collection | OXFORD |
| description | Melioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in blood usually falls below the achievable level of detection. We performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to detection of B. pseudomallei using a monoclonal antibody-based immunofluorescent assay (Mab-IFA). The Mab-IFA was positive in 74 of 76 patients with melioidosis (sensitivity = 97.4%), and negative in 385 patients who did not have blood cultures containing B. pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic. |
| first_indexed | 2024-03-06T20:11:57Z |
| format | Journal article |
| id | oxford-uuid:2adf24ef-3271-42b5-b94a-22cd21c296f9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:11:57Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:2adf24ef-3271-42b5-b94a-22cd21c296f92022-03-26T12:27:34ZRapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2adf24ef-3271-42b5-b94a-22cd21c296f9EnglishSymplectic Elements at Oxford2013Chantratita, NTandhavanant, SWongsuvan, GWuthiekanun, VTeerawattanasook, NDay, NLimmathurotsakul, DPeacock, SMelioidosis is a severe bacterial infection caused by Burkholderia pseudomallei. Rapid antimicrobial therapy is necessary to improve patient outcome, which is aided by direct detection of B. pseudomallei in clinical samples. A drawback for all antigen assays is that the number of B. pseudomallei in blood usually falls below the achievable level of detection. We performed a prospective cohort study of 461 patients with 541 blood cultures to evaluate the utility of a pre-incubation step prior to detection of B. pseudomallei using a monoclonal antibody-based immunofluorescent assay (Mab-IFA). The Mab-IFA was positive in 74 of 76 patients with melioidosis (sensitivity = 97.4%), and negative in 385 patients who did not have blood cultures containing B. pseudomallei (specificity = 100%). The Mab-IFA could be a valuable supplementary tool for rapid detection. We recommend the use of the Mab-IFA to test blood cultures that flag positive in regions where melioidosis is endemic. |
| spellingShingle | Chantratita, N Tandhavanant, S Wongsuvan, G Wuthiekanun, V Teerawattanasook, N Day, N Limmathurotsakul, D Peacock, S Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. |
| title | Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. |
| title_full | Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. |
| title_fullStr | Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. |
| title_full_unstemmed | Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. |
| title_short | Rapid detection of Burkholderia pseudomallei in blood cultures using a monoclonal antibody-based immunofluorescent assay. |
| title_sort | rapid detection of burkholderia pseudomallei in blood cultures using a monoclonal antibody based immunofluorescent assay |
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