Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart.
This study examined cardiac function and glucose metabolism in the 6-month-old db/db mouse, a model of type-2 diabetes. Cine magnetic resonance spectroscopy (MRI) was used to measure cardiac function in vivo. The db/db mice had decreased heart rates (17%, p<0.01) and stroke volumes (21%, p&am...
| Main Authors: | , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2007
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| _version_ | 1826264764722970624 |
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| author | Panagia, M Schneider, J Brown, B Cole, M Clarke, K |
| author_facet | Panagia, M Schneider, J Brown, B Cole, M Clarke, K |
| author_sort | Panagia, M |
| collection | OXFORD |
| description | This study examined cardiac function and glucose metabolism in the 6-month-old db/db mouse, a model of type-2 diabetes. Cine magnetic resonance spectroscopy (MRI) was used to measure cardiac function in vivo. The db/db mice had decreased heart rates (17%, p<0.01) and stroke volumes (21%, p<0.05) that resulted in lower cardiac output (35%, p<0.01) than controls. Although there was no difference in ejection fraction between the 2 groups, db/db mouse hearts had a 35% lower maximum rate of ejection (p<0.01) than controls. In a protocol designed to assess maximal insulin-independent glucose uptake, hearts were isolated and perfused in Langendorff mode and subjected to 0.75 mL.min(-1).(g wet mass)(-1) low flow ischemia for 32 min. Glucose uptake during ischemia was 21% lower than in controls, and post-ischemic recovery of cardiac function was decreased by 30% in db/db mouse hearts (p<0.05). Total cardiac GLUT 4 protein was 56% lower (p<0.01) in db/db mice than in controls. In summary, the db/db mouse has abnormal left ventricular function in vivo, with impaired glucose uptake during ischemia, leading to increased myocardial damage. |
| first_indexed | 2024-03-06T20:13:07Z |
| format | Journal article |
| id | oxford-uuid:2b42b4ac-c364-4757-b20e-f1dd2add877d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:13:07Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:2b42b4ac-c364-4757-b20e-f1dd2add877d2022-03-26T12:29:48ZAbnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2b42b4ac-c364-4757-b20e-f1dd2add877dEnglishSymplectic Elements at Oxford2007Panagia, MSchneider, JBrown, BCole, MClarke, KThis study examined cardiac function and glucose metabolism in the 6-month-old db/db mouse, a model of type-2 diabetes. Cine magnetic resonance spectroscopy (MRI) was used to measure cardiac function in vivo. The db/db mice had decreased heart rates (17%, p<0.01) and stroke volumes (21%, p<0.05) that resulted in lower cardiac output (35%, p<0.01) than controls. Although there was no difference in ejection fraction between the 2 groups, db/db mouse hearts had a 35% lower maximum rate of ejection (p<0.01) than controls. In a protocol designed to assess maximal insulin-independent glucose uptake, hearts were isolated and perfused in Langendorff mode and subjected to 0.75 mL.min(-1).(g wet mass)(-1) low flow ischemia for 32 min. Glucose uptake during ischemia was 21% lower than in controls, and post-ischemic recovery of cardiac function was decreased by 30% in db/db mouse hearts (p<0.05). Total cardiac GLUT 4 protein was 56% lower (p<0.01) in db/db mice than in controls. In summary, the db/db mouse has abnormal left ventricular function in vivo, with impaired glucose uptake during ischemia, leading to increased myocardial damage. |
| spellingShingle | Panagia, M Schneider, J Brown, B Cole, M Clarke, K Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart. |
| title | Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart. |
| title_full | Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart. |
| title_fullStr | Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart. |
| title_full_unstemmed | Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart. |
| title_short | Abnormal function and glucose metabolism in the type-2 diabetic db/db mouse heart. |
| title_sort | abnormal function and glucose metabolism in the type 2 diabetic db db mouse heart |
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