Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs.
The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base...
| Asıl Yazarlar: | , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
1989
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| _version_ | 1826264793237946368 |
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| author | Brown, T Leonard, G Booth, E Chambers, J |
| author_facet | Brown, T Leonard, G Booth, E Chambers, J |
| author_sort | Brown, T |
| collection | OXFORD |
| description | The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis clearly shows that the mismatches are of the form A(anti).G(syn). Thermal denaturation studies indicate that the stability of the duplex is strongly pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is stabilized by protonation. Three different arrangements have been observed for base-pairs between guanine and adenine and it is likely that A.G mismatch conformation is strongly influenced by dipole-dipole interactions with adjacent base-pairs. |
| first_indexed | 2024-03-06T20:13:33Z |
| format | Journal article |
| id | oxford-uuid:2b63b88e-2ed5-43f5-a476-b44cd72a4b15 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:13:33Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:2b63b88e-2ed5-43f5-a476-b44cd72a4b152022-03-26T12:30:39ZCrystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2b63b88e-2ed5-43f5-a476-b44cd72a4b15EnglishSymplectic Elements at Oxford1989Brown, TLeonard, GBooth, EChambers, JThe synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis clearly shows that the mismatches are of the form A(anti).G(syn). Thermal denaturation studies indicate that the stability of the duplex is strongly pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is stabilized by protonation. Three different arrangements have been observed for base-pairs between guanine and adenine and it is likely that A.G mismatch conformation is strongly influenced by dipole-dipole interactions with adjacent base-pairs. |
| spellingShingle | Brown, T Leonard, G Booth, E Chambers, J Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs. |
| title | Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs. |
| title_full | Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs. |
| title_fullStr | Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs. |
| title_full_unstemmed | Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs. |
| title_short | Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs. |
| title_sort | crystal structure and stability of a dna duplex containing a anti g syn base pairs |
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