Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin.
This protocol describes a simple and efficient way to label specific cell surface proteins with biophysical probes on mammalian cells. Cell surface proteins tagged with a 15-amino acid peptide are biotinylated by Escherichia coli biotin ligase (BirA), whereas endogenous proteins are not modified. Th...
| Main Authors: | , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado: |
2008
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