Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin.

Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin.

This protocol describes a simple and efficient way to label specific cell surface proteins with biophysical probes on mammalian cells. Cell surface proteins tagged with a 15-amino acid peptide are biotinylated by Escherichia coli biotin ligase (BirA), whereas endogenous proteins are not modified. Th...

書誌詳細
主要な著者:	Howarth, M, Ting, A
フォーマット:	Journal article
言語:	English
出版事項:	2008

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