Accuracy and reproducibility of protein-DNA microarray technology.
Microarray-based methods for understanding protein-DNA interactions have been developed in the last 6 years due to the need to introduce high-throughput technologies in this field. Protein-DNA microarrays utilise chips upon which a large number of DNA sequences may be printed or synthesised. Any DNA...
| Հիմնական հեղինակներ: | , , |
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| Ձևաչափ: | Journal article |
| Լեզու: | English |
| Հրապարակվել է: |
2007
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| _version_ | 1826264825773162496 |
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| author | Field, S Udalova, I Ragoussis, J |
| author_facet | Field, S Udalova, I Ragoussis, J |
| author_sort | Field, S |
| collection | OXFORD |
| description | Microarray-based methods for understanding protein-DNA interactions have been developed in the last 6 years due to the need to introduce high-throughput technologies in this field. Protein-DNA microarrays utilise chips upon which a large number of DNA sequences may be printed or synthesised. Any DNA-binding protein may then be interrogated by applying either purified sample or cellular/nuclear extracts, subject to availability of a suitable detection system. Protein is simply added to the microarray slide surface, which is then washed and subjected to at least one further incubation with a labelled molecule which binds specifically to the protein of interest. The signal obtained is proportional to the level of DNA-binding protein bound to each DNA feature, enabling relative affinities to be calculated. Key factors for reproducible and accurate quantification of protein binding are: microarray surface chemistry; length of oligonucleotides; position of the binding site sequence; quality of the protein and antibodies; and hybridisation conditions. |
| first_indexed | 2024-03-06T20:14:04Z |
| format | Journal article |
| id | oxford-uuid:2b8dca8e-175c-4097-9c8b-16fdb7d63d70 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:14:04Z |
| publishDate | 2007 |
| record_format | dspace |
| spelling | oxford-uuid:2b8dca8e-175c-4097-9c8b-16fdb7d63d702022-03-26T12:31:37ZAccuracy and reproducibility of protein-DNA microarray technology.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2b8dca8e-175c-4097-9c8b-16fdb7d63d70EnglishSymplectic Elements at Oxford2007Field, SUdalova, IRagoussis, JMicroarray-based methods for understanding protein-DNA interactions have been developed in the last 6 years due to the need to introduce high-throughput technologies in this field. Protein-DNA microarrays utilise chips upon which a large number of DNA sequences may be printed or synthesised. Any DNA-binding protein may then be interrogated by applying either purified sample or cellular/nuclear extracts, subject to availability of a suitable detection system. Protein is simply added to the microarray slide surface, which is then washed and subjected to at least one further incubation with a labelled molecule which binds specifically to the protein of interest. The signal obtained is proportional to the level of DNA-binding protein bound to each DNA feature, enabling relative affinities to be calculated. Key factors for reproducible and accurate quantification of protein binding are: microarray surface chemistry; length of oligonucleotides; position of the binding site sequence; quality of the protein and antibodies; and hybridisation conditions. |
| spellingShingle | Field, S Udalova, I Ragoussis, J Accuracy and reproducibility of protein-DNA microarray technology. |
| title | Accuracy and reproducibility of protein-DNA microarray technology. |
| title_full | Accuracy and reproducibility of protein-DNA microarray technology. |
| title_fullStr | Accuracy and reproducibility of protein-DNA microarray technology. |
| title_full_unstemmed | Accuracy and reproducibility of protein-DNA microarray technology. |
| title_short | Accuracy and reproducibility of protein-DNA microarray technology. |
| title_sort | accuracy and reproducibility of protein dna microarray technology |
| work_keys_str_mv | AT fields accuracyandreproducibilityofproteindnamicroarraytechnology AT udalovai accuracyandreproducibilityofproteindnamicroarraytechnology AT ragoussisj accuracyandreproducibilityofproteindnamicroarraytechnology |