The power and prospects of fluorescence microscopies and spectroscopies.
Recent years have witnessed a renaissance of fluorescence microscopy techniques and applications, from live-animal multiphoton confocal microscopy to single-molecule fluorescence spectroscopy and imaging in living cells. These achievements have been made possible not so much because of improvements...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1797060312201953280 |
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| author | Michalet, X Kapanidis, A Laurence, T Pinaud, F Doose, S Pflughoefft, M Weiss, S |
| author_facet | Michalet, X Kapanidis, A Laurence, T Pinaud, F Doose, S Pflughoefft, M Weiss, S |
| author_sort | Michalet, X |
| collection | OXFORD |
| description | Recent years have witnessed a renaissance of fluorescence microscopy techniques and applications, from live-animal multiphoton confocal microscopy to single-molecule fluorescence spectroscopy and imaging in living cells. These achievements have been made possible not so much because of improvements in microscope design, but rather because of development of new detectors, accessible continuous wave and pulsed laser sources, sophisticated multiparameter analysis on one hand, and the development of new probes and labeling chemistries on the other. This review tracks the lineage of ideas and the evolution of thinking that have led to the actual developments, and presents a comprehensive overview of the field, with emphasis put on our laboratory's interest in single-molecule microscopy and spectroscopy. |
| first_indexed | 2024-03-06T20:15:22Z |
| format | Journal article |
| id | oxford-uuid:2bf73dc0-bde5-4a4a-af67-97c3df7b6399 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:15:22Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:2bf73dc0-bde5-4a4a-af67-97c3df7b63992022-03-26T12:34:10ZThe power and prospects of fluorescence microscopies and spectroscopies.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2bf73dc0-bde5-4a4a-af67-97c3df7b6399EnglishSymplectic Elements at Oxford2003Michalet, XKapanidis, ALaurence, TPinaud, FDoose, SPflughoefft, MWeiss, SRecent years have witnessed a renaissance of fluorescence microscopy techniques and applications, from live-animal multiphoton confocal microscopy to single-molecule fluorescence spectroscopy and imaging in living cells. These achievements have been made possible not so much because of improvements in microscope design, but rather because of development of new detectors, accessible continuous wave and pulsed laser sources, sophisticated multiparameter analysis on one hand, and the development of new probes and labeling chemistries on the other. This review tracks the lineage of ideas and the evolution of thinking that have led to the actual developments, and presents a comprehensive overview of the field, with emphasis put on our laboratory's interest in single-molecule microscopy and spectroscopy. |
| spellingShingle | Michalet, X Kapanidis, A Laurence, T Pinaud, F Doose, S Pflughoefft, M Weiss, S The power and prospects of fluorescence microscopies and spectroscopies. |
| title | The power and prospects of fluorescence microscopies and spectroscopies. |
| title_full | The power and prospects of fluorescence microscopies and spectroscopies. |
| title_fullStr | The power and prospects of fluorescence microscopies and spectroscopies. |
| title_full_unstemmed | The power and prospects of fluorescence microscopies and spectroscopies. |
| title_short | The power and prospects of fluorescence microscopies and spectroscopies. |
| title_sort | power and prospects of fluorescence microscopies and spectroscopies |
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