Image-based adaptive optics for two-photon microscopy.
We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that...
| Huvudupphovsmän: | , , , , , |
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| Materialtyp: | Journal article |
| Språk: | English |
| Publicerad: |
2009
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| _version_ | 1826264921685360640 |
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| author | Débarre, D Botcherby, E Watanabe, T Srinivas, S Booth, M Wilson, T |
| author_facet | Débarre, D Botcherby, E Watanabe, T Srinivas, S Booth, M Wilson, T |
| author_sort | Débarre, D |
| collection | OXFORD |
| description | We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues. |
| first_indexed | 2024-03-06T20:15:34Z |
| format | Journal article |
| id | oxford-uuid:2c099559-2e4b-427d-bbe6-c0183692dfc2 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:15:34Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:2c099559-2e4b-427d-bbe6-c0183692dfc22022-03-26T12:34:34ZImage-based adaptive optics for two-photon microscopy.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2c099559-2e4b-427d-bbe6-c0183692dfc2EnglishSymplectic Elements at Oxford2009Débarre, DBotcherby, EWatanabe, TSrinivas, SBooth, MWilson, TWe demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that, as a result, our correction process induces little photobleaching and significantly improves the quality of images of biological samples. In particular, increased visibility of small structures is demonstrated. Finally, we illustrate the use of this technique on various fresh and fixed biological tissues. |
| spellingShingle | Débarre, D Botcherby, E Watanabe, T Srinivas, S Booth, M Wilson, T Image-based adaptive optics for two-photon microscopy. |
| title | Image-based adaptive optics for two-photon microscopy. |
| title_full | Image-based adaptive optics for two-photon microscopy. |
| title_fullStr | Image-based adaptive optics for two-photon microscopy. |
| title_full_unstemmed | Image-based adaptive optics for two-photon microscopy. |
| title_short | Image-based adaptive optics for two-photon microscopy. |
| title_sort | image based adaptive optics for two photon microscopy |
| work_keys_str_mv | AT debarred imagebasedadaptiveopticsfortwophotonmicroscopy AT botcherbye imagebasedadaptiveopticsfortwophotonmicroscopy AT watanabet imagebasedadaptiveopticsfortwophotonmicroscopy AT srinivass imagebasedadaptiveopticsfortwophotonmicroscopy AT boothm imagebasedadaptiveopticsfortwophotonmicroscopy AT wilsont imagebasedadaptiveopticsfortwophotonmicroscopy |