Image-based adaptive optics for two-photon microscopy.

We demonstrate wavefront sensorless aberration correction in a two-photon excited fluorescence microscope. Using analysis of the image-formation process, we have developed an optimized correction scheme permitting image-quality improvement with minimal additional exposure of the sample. We show that...

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Bibliographic Details
Main Authors: Débarre, D, Botcherby, E, Watanabe, T, Srinivas, S, Booth, M, Wilson, T
Format: Journal article
Language:English
Published: 2009