CD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling.
The transmembrane phosphatase CD45 regulates both Lck activity and T cell receptor (TCR) signaling. Here we have tested whether the large ectodomain of CD45 has a role in this regulation. A CD45 chimera containing the large ectodomain of CD43 efficiently rescues TCR signaling in CD45-null T cells, w...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1797060353503264768 |
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| author | Irles, C Symons, A Michel, F Bakker, T Van Der Merwe, P Acuto, O |
| author_facet | Irles, C Symons, A Michel, F Bakker, T Van Der Merwe, P Acuto, O |
| author_sort | Irles, C |
| collection | OXFORD |
| description | The transmembrane phosphatase CD45 regulates both Lck activity and T cell receptor (TCR) signaling. Here we have tested whether the large ectodomain of CD45 has a role in this regulation. A CD45 chimera containing the large ectodomain of CD43 efficiently rescues TCR signaling in CD45-null T cells, whereas CD45 chimeras containing small ectodomains from other phosphatases do not. Both basal Lck activity in unstimulated cells and the TCR-induced increase in tyrosine phosphorylation of the TCR zeta-chain and in Lck activity depend on the expression of CD45 with a large ectodomain. Unlike CD45 chimeras containing small ectodomains, both the CD45 chimera with a large ectodomain and wild-type CD45 itself are partially localized to glycosphingolipid-enriched membranes (GEMs). Taken together, these data show that the large CD45 ectodomain is required for optimal TCR signaling. |
| first_indexed | 2024-03-06T20:15:55Z |
| format | Journal article |
| id | oxford-uuid:2c23401a-44d8-4bbf-bf79-b342602e016d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:15:55Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:2c23401a-44d8-4bbf-bf79-b342602e016d2022-03-26T12:35:09ZCD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2c23401a-44d8-4bbf-bf79-b342602e016dEnglishSymplectic Elements at Oxford2003Irles, CSymons, AMichel, FBakker, TVan Der Merwe, PAcuto, OThe transmembrane phosphatase CD45 regulates both Lck activity and T cell receptor (TCR) signaling. Here we have tested whether the large ectodomain of CD45 has a role in this regulation. A CD45 chimera containing the large ectodomain of CD43 efficiently rescues TCR signaling in CD45-null T cells, whereas CD45 chimeras containing small ectodomains from other phosphatases do not. Both basal Lck activity in unstimulated cells and the TCR-induced increase in tyrosine phosphorylation of the TCR zeta-chain and in Lck activity depend on the expression of CD45 with a large ectodomain. Unlike CD45 chimeras containing small ectodomains, both the CD45 chimera with a large ectodomain and wild-type CD45 itself are partially localized to glycosphingolipid-enriched membranes (GEMs). Taken together, these data show that the large CD45 ectodomain is required for optimal TCR signaling. |
| spellingShingle | Irles, C Symons, A Michel, F Bakker, T Van Der Merwe, P Acuto, O CD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling. |
| title | CD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling. |
| title_full | CD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling. |
| title_fullStr | CD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling. |
| title_full_unstemmed | CD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling. |
| title_short | CD45 ectodomain controls interaction with GEMs and Lck activity for optimal TCR signaling. |
| title_sort | cd45 ectodomain controls interaction with gems and lck activity for optimal tcr signaling |
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