Mass spectrometry guided structural biology.
With the convergence of breakthroughs in structural biology, specifically breaking the resolution barriers in cryo-electron microscopy and with continuing developments in crystallography, novel interfaces with other biophysical methods are emerging. Here we consider how mass spectrometry can inform...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2016
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| Subjects: |
| _version_ | 1826265144021221376 |
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| author | Liko, I Allison, T Hopper, J Robinson, C |
| author_facet | Liko, I Allison, T Hopper, J Robinson, C |
| author_sort | Liko, I |
| collection | OXFORD |
| description | With the convergence of breakthroughs in structural biology, specifically breaking the resolution barriers in cryo-electron microscopy and with continuing developments in crystallography, novel interfaces with other biophysical methods are emerging. Here we consider how mass spectrometry can inform these techniques by providing unambiguous definition of subunit stoichiometry. Moreover recent developments that increase mass spectral resolution enable molecular details to be ascribed to unassigned density within high-resolution maps of membrane and soluble protein complexes. Importantly we also show how developments in mass spectrometry can define optimal solution conditions to guide downstream structure determination, particularly of challenging biomolecules that refuse to crystallise. |
| first_indexed | 2024-03-06T20:19:03Z |
| format | Journal article |
| id | oxford-uuid:2d2e6697-264a-40a5-8c42-5e688e17bd82 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:19:03Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:2d2e6697-264a-40a5-8c42-5e688e17bd822022-03-26T12:41:14ZMass spectrometry guided structural biology.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2d2e6697-264a-40a5-8c42-5e688e17bd82*subject*EnglishSymplectic Elements at OxfordElsevier2016Liko, IAllison, THopper, JRobinson, CWith the convergence of breakthroughs in structural biology, specifically breaking the resolution barriers in cryo-electron microscopy and with continuing developments in crystallography, novel interfaces with other biophysical methods are emerging. Here we consider how mass spectrometry can inform these techniques by providing unambiguous definition of subunit stoichiometry. Moreover recent developments that increase mass spectral resolution enable molecular details to be ascribed to unassigned density within high-resolution maps of membrane and soluble protein complexes. Importantly we also show how developments in mass spectrometry can define optimal solution conditions to guide downstream structure determination, particularly of challenging biomolecules that refuse to crystallise. |
| spellingShingle | *subject* Liko, I Allison, T Hopper, J Robinson, C Mass spectrometry guided structural biology. |
| title | Mass spectrometry guided structural biology. |
| title_full | Mass spectrometry guided structural biology. |
| title_fullStr | Mass spectrometry guided structural biology. |
| title_full_unstemmed | Mass spectrometry guided structural biology. |
| title_short | Mass spectrometry guided structural biology. |
| title_sort | mass spectrometry guided structural biology |
| topic | *subject* |
| work_keys_str_mv | AT likoi massspectrometryguidedstructuralbiology AT allisont massspectrometryguidedstructuralbiology AT hopperj massspectrometryguidedstructuralbiology AT robinsonc massspectrometryguidedstructuralbiology |