MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines.
For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2009
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| _version_ | 1797060607594201088 |
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| author | Berthoud, T Dunachie, S Todryk, S Hill, A Fletcher, H |
| author_facet | Berthoud, T Dunachie, S Todryk, S Hill, A Fletcher, H |
| author_sort | Berthoud, T |
| collection | OXFORD |
| description | For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination. |
| first_indexed | 2024-03-06T20:19:28Z |
| format | Journal article |
| id | oxford-uuid:2d4bfa85-161d-4d82-ae12-8df797c51d8e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:19:28Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:2d4bfa85-161d-4d82-ae12-8df797c51d8e2022-03-26T12:42:06ZMIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2d4bfa85-161d-4d82-ae12-8df797c51d8eEnglishSymplectic Elements at Oxford2009Berthoud, TDunachie, STodryk, SHill, AFletcher, HFor many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination. |
| spellingShingle | Berthoud, T Dunachie, S Todryk, S Hill, A Fletcher, H MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines. |
| title | MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines. |
| title_full | MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines. |
| title_fullStr | MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines. |
| title_full_unstemmed | MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines. |
| title_short | MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines. |
| title_sort | mig cxcl9 is a more sensitive measure than ifn gamma of vaccine induced t cell responses in volunteers receiving investigated malaria vaccines |
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