A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture.
This study reports a new perfusion-based, micro three-dimensional (3-D) cell culture platform for high-throughput cell culture using enabling microfluidic technologies. In this work, the micro 3-D cell culture platform is fabricated based on SU-8 lithography and polydimethylsiloxane replication proc...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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2008
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| _version_ | 1797060877415874560 |
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| author | Wu, M Huang, S Cui, Z Cui, Z Lee, G |
| author_facet | Wu, M Huang, S Cui, Z Cui, Z Lee, G |
| author_sort | Wu, M |
| collection | OXFORD |
| description | This study reports a new perfusion-based, micro three-dimensional (3-D) cell culture platform for high-throughput cell culture using enabling microfluidic technologies. In this work, the micro 3-D cell culture platform is fabricated based on SU-8 lithography and polydimethylsiloxane replication processes. The micro cell culture platform can maintain homogenous and stable culture environments, as well as provide pumping of multiple mediums and efficient cell/agarose (scaffold) loading functions, which allows realization of more precise and high-throughput cell culture-based assays. In this study, the design of a high-throughput medium pumping mechanism was especially highlighted. A new serpentine-shaped pneumatic micropump was used to provide the required medium pumping mechanism. Pneumatic microchannels with a varied length and U-shape bending corners were designed to connect three rectangular pneumatic chambers such that one can fine-tune the pumping rate of the S-shape micropump by using the fluidic resistance. To achieve a high-throughput medium pumping function, a pneumatic tank was designed to simultaneously activate all of the 30 pneumatic micropumps with a uniform pumping rate. Results show that the pumping rates of the 30 integrated micropumps were statistically uniform with a flow rate ranging from 8.5 to 185.1 microl h(-1), indicating the present multiple medium pumping mechanism is feasible for high-throughput medium delivery purposes. Furthermore, as a demonstration case study, 3-D culture of oral cancer cell was successfully performed, showing that the cell viability remained as high as 95% - 98% during the 48 h cell culture. As the result of miniaturization, this perfusion-based 3-D cell culture platform not only provides a well-defined and stable culture condition, but also greatly reduces the sample/reagent consumption and the need for human intervention. Moreover, due to the integrated capability for multiple medium pumping, high-throughput research work can be achieved. These traits are found particularly useful for high-precision and high-throughput, 3-D cell culture-based assay. |
| first_indexed | 2024-03-06T20:23:09Z |
| format | Journal article |
| id | oxford-uuid:2e824034-2ac7-43b4-a247-fe0fa982eef4 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:23:09Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:2e824034-2ac7-43b4-a247-fe0fa982eef42022-03-26T12:49:20ZA high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2e824034-2ac7-43b4-a247-fe0fa982eef4EnglishSymplectic Elements at Oxford2008Wu, MHuang, SCui, ZCui, ZLee, GThis study reports a new perfusion-based, micro three-dimensional (3-D) cell culture platform for high-throughput cell culture using enabling microfluidic technologies. In this work, the micro 3-D cell culture platform is fabricated based on SU-8 lithography and polydimethylsiloxane replication processes. The micro cell culture platform can maintain homogenous and stable culture environments, as well as provide pumping of multiple mediums and efficient cell/agarose (scaffold) loading functions, which allows realization of more precise and high-throughput cell culture-based assays. In this study, the design of a high-throughput medium pumping mechanism was especially highlighted. A new serpentine-shaped pneumatic micropump was used to provide the required medium pumping mechanism. Pneumatic microchannels with a varied length and U-shape bending corners were designed to connect three rectangular pneumatic chambers such that one can fine-tune the pumping rate of the S-shape micropump by using the fluidic resistance. To achieve a high-throughput medium pumping function, a pneumatic tank was designed to simultaneously activate all of the 30 pneumatic micropumps with a uniform pumping rate. Results show that the pumping rates of the 30 integrated micropumps were statistically uniform with a flow rate ranging from 8.5 to 185.1 microl h(-1), indicating the present multiple medium pumping mechanism is feasible for high-throughput medium delivery purposes. Furthermore, as a demonstration case study, 3-D culture of oral cancer cell was successfully performed, showing that the cell viability remained as high as 95% - 98% during the 48 h cell culture. As the result of miniaturization, this perfusion-based 3-D cell culture platform not only provides a well-defined and stable culture condition, but also greatly reduces the sample/reagent consumption and the need for human intervention. Moreover, due to the integrated capability for multiple medium pumping, high-throughput research work can be achieved. These traits are found particularly useful for high-precision and high-throughput, 3-D cell culture-based assay. |
| spellingShingle | Wu, M Huang, S Cui, Z Cui, Z Lee, G A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture. |
| title | A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture. |
| title_full | A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture. |
| title_fullStr | A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture. |
| title_full_unstemmed | A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture. |
| title_short | A high throughput perfusion-based microbioreactor platform integrated with pneumatic micropumps for three-dimensional cell culture. |
| title_sort | high throughput perfusion based microbioreactor platform integrated with pneumatic micropumps for three dimensional cell culture |
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