Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism.
Adaptive responses to hypoxia occur in many biological systems. A well-characterized example is the hypoxic induction of the synthesis of erythropoietin, a hormone which regulates erythropoiesis and hence blood oxygen content. The restricted expression of the erythropoietin gene in subsets of cells...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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1993
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| _version_ | 1797060878651097088 |
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| author | Maxwell, P Pugh, C Ratcliffe, P |
| author_facet | Maxwell, P Pugh, C Ratcliffe, P |
| author_sort | Maxwell, P |
| collection | OXFORD |
| description | Adaptive responses to hypoxia occur in many biological systems. A well-characterized example is the hypoxic induction of the synthesis of erythropoietin, a hormone which regulates erythropoiesis and hence blood oxygen content. The restricted expression of the erythropoietin gene in subsets of cells within kidney and liver has suggested that this specific oxygen-sensing mechanism is restricted to specialized cells in those organs. Using transient transfection of reporter genes coupled to a transcriptional enhancer lying 3' to the erythropoietin gene, we show that an oxygen-sensing system similar, or identical, to that controlling erythropoietin expression is wide-spread in mammalian cells. The extensive distribution of this sensing mechanism contrasts with the restricted expression of erythropoietin, suggesting that it mediates other adaptive responses to hypoxia. |
| first_indexed | 2024-03-06T20:23:10Z |
| format | Journal article |
| id | oxford-uuid:2e839bb3-4ad8-4e1d-bf09-e208a8774b8f |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:23:10Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | oxford-uuid:2e839bb3-4ad8-4e1d-bf09-e208a8774b8f2022-03-26T12:49:23ZInducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2e839bb3-4ad8-4e1d-bf09-e208a8774b8fEnglishSymplectic Elements at Oxford1993Maxwell, PPugh, CRatcliffe, PAdaptive responses to hypoxia occur in many biological systems. A well-characterized example is the hypoxic induction of the synthesis of erythropoietin, a hormone which regulates erythropoiesis and hence blood oxygen content. The restricted expression of the erythropoietin gene in subsets of cells within kidney and liver has suggested that this specific oxygen-sensing mechanism is restricted to specialized cells in those organs. Using transient transfection of reporter genes coupled to a transcriptional enhancer lying 3' to the erythropoietin gene, we show that an oxygen-sensing system similar, or identical, to that controlling erythropoietin expression is wide-spread in mammalian cells. The extensive distribution of this sensing mechanism contrasts with the restricted expression of erythropoietin, suggesting that it mediates other adaptive responses to hypoxia. |
| spellingShingle | Maxwell, P Pugh, C Ratcliffe, P Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism. |
| title | Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism. |
| title_full | Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism. |
| title_fullStr | Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism. |
| title_full_unstemmed | Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism. |
| title_short | Inducible operation of the erythropoietin 3' enhancer in multiple cell lines: evidence for a widespread oxygen-sensing mechanism. |
| title_sort | inducible operation of the erythropoietin 3 enhancer in multiple cell lines evidence for a widespread oxygen sensing mechanism |
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