Evaluation of the diagnostic accuracy of a typhoid IgM flow assay for the diagnosis of typhoid fever in Cambodian children using a Bayesian latent class model assuming an imperfect gold standard
<p>Rapid diagnostic tests are needed for typhoid fever (TF) diagnosis in febrile children in endemic areas. Five hundred children admitted to the hospital in Cambodia between 2009 and 2010 with documented fever (≥ 38°C) were investigated using blood cultures (BCs), <em>Salmonella</em&...
| Príomhchruthaitheoirí: | , , , , , , , , , , , , , , |
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| Formáid: | Journal article |
| Teanga: | English |
| Foilsithe / Cruthaithe: |
American Society of Tropical Medicine and Hygiene
2014
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| Ábhair: |
| _version_ | 1826265476761649152 |
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| author | Moore, C Pan-Ngum, W Wijedoru, L Sona, S Nga, T Duy, P Vinh, P Chheng, K Kumar, V Emary, K Carter, M White, L Baker, S Day, N Parry, C |
| author_facet | Moore, C Pan-Ngum, W Wijedoru, L Sona, S Nga, T Duy, P Vinh, P Chheng, K Kumar, V Emary, K Carter, M White, L Baker, S Day, N Parry, C |
| author_sort | Moore, C |
| collection | OXFORD |
| description | <p>Rapid diagnostic tests are needed for typhoid fever (TF) diagnosis in febrile children in endemic areas. Five hundred children admitted to the hospital in Cambodia between 2009 and 2010 with documented fever (≥ 38°C) were investigated using blood cultures (BCs), <em>Salmonella</em> Typhi/Paratyphi A real-time polymerase chain reactions (PCRs), and a Typhoid immunoglobulin M flow assays (IgMFAs). Test performance was determined by conventional methods and Bayesian latent class modeling. There were 32 cases of TF (10 BC- and PCR-positive cases, 14 BC-positive and PCR-negative cases, and 8 BCnegative and PCR-positive cases). IgMFA sensitivity was 59.4% (95% confidence interval = 41–76), and specificity WAS 97.8% (95% confidence interval = 96–99). The model estimate sensitivity for BC was 81.0% (95% credible interval = 54–99). The model estimate sensitivity for PCR was 37.8% (95% credible interval = 26–55), with a specificity of 98.2% (95% credible interval = 97–99). The model estimate sensitivity for IgMFA (≥ 2+) was 77.9% (95% credible interval = 58–90), with a specificity of 97.5% (95% credible interval = 95–100). The model estimates of IgMFA sensitivity and specificity were comparable with BCs and better than estimates using conventional analysis.</p> |
| first_indexed | 2024-03-06T20:24:18Z |
| format | Journal article |
| id | oxford-uuid:2ee3eb9e-a83f-4024-b37c-b706afb7adf5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:24:18Z |
| publishDate | 2014 |
| publisher | American Society of Tropical Medicine and Hygiene |
| record_format | dspace |
| spelling | oxford-uuid:2ee3eb9e-a83f-4024-b37c-b706afb7adf52022-03-26T12:51:42ZEvaluation of the diagnostic accuracy of a typhoid IgM flow assay for the diagnosis of typhoid fever in Cambodian children using a Bayesian latent class model assuming an imperfect gold standardJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2ee3eb9e-a83f-4024-b37c-b706afb7adf5EpidemiologyPaediatricsClinical microbiologyTropical medicineInfectious diseasesEnglishOxford University Research Archive - ValetAmerican Society of Tropical Medicine and Hygiene2014Moore, CPan-Ngum, WWijedoru, LSona, SNga, TDuy, PVinh, PChheng, KKumar, VEmary, KCarter, MWhite, LBaker, SDay, NParry, C<p>Rapid diagnostic tests are needed for typhoid fever (TF) diagnosis in febrile children in endemic areas. Five hundred children admitted to the hospital in Cambodia between 2009 and 2010 with documented fever (≥ 38°C) were investigated using blood cultures (BCs), <em>Salmonella</em> Typhi/Paratyphi A real-time polymerase chain reactions (PCRs), and a Typhoid immunoglobulin M flow assays (IgMFAs). Test performance was determined by conventional methods and Bayesian latent class modeling. There were 32 cases of TF (10 BC- and PCR-positive cases, 14 BC-positive and PCR-negative cases, and 8 BCnegative and PCR-positive cases). IgMFA sensitivity was 59.4% (95% confidence interval = 41–76), and specificity WAS 97.8% (95% confidence interval = 96–99). The model estimate sensitivity for BC was 81.0% (95% credible interval = 54–99). The model estimate sensitivity for PCR was 37.8% (95% credible interval = 26–55), with a specificity of 98.2% (95% credible interval = 97–99). The model estimate sensitivity for IgMFA (≥ 2+) was 77.9% (95% credible interval = 58–90), with a specificity of 97.5% (95% credible interval = 95–100). The model estimates of IgMFA sensitivity and specificity were comparable with BCs and better than estimates using conventional analysis.</p> |
| spellingShingle | Epidemiology Paediatrics Clinical microbiology Tropical medicine Infectious diseases Moore, C Pan-Ngum, W Wijedoru, L Sona, S Nga, T Duy, P Vinh, P Chheng, K Kumar, V Emary, K Carter, M White, L Baker, S Day, N Parry, C Evaluation of the diagnostic accuracy of a typhoid IgM flow assay for the diagnosis of typhoid fever in Cambodian children using a Bayesian latent class model assuming an imperfect gold standard |
| title | Evaluation of the diagnostic accuracy of a typhoid IgM flow assay for the diagnosis of typhoid fever in Cambodian children using a Bayesian latent class model assuming an imperfect gold standard |
| title_full | Evaluation of the diagnostic accuracy of a typhoid IgM flow assay for the diagnosis of typhoid fever in Cambodian children using a Bayesian latent class model assuming an imperfect gold standard |
| title_fullStr | Evaluation of the diagnostic accuracy of a typhoid IgM flow assay for the diagnosis of typhoid fever in Cambodian children using a Bayesian latent class model assuming an imperfect gold standard |
| title_full_unstemmed | Evaluation of the diagnostic accuracy of a typhoid IgM flow assay for the diagnosis of typhoid fever in Cambodian children using a Bayesian latent class model assuming an imperfect gold standard |
| title_short | Evaluation of the diagnostic accuracy of a typhoid IgM flow assay for the diagnosis of typhoid fever in Cambodian children using a Bayesian latent class model assuming an imperfect gold standard |
| title_sort | evaluation of the diagnostic accuracy of a typhoid igm flow assay for the diagnosis of typhoid fever in cambodian children using a bayesian latent class model assuming an imperfect gold standard |
| topic | Epidemiology Paediatrics Clinical microbiology Tropical medicine Infectious diseases |
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