USP7/HAUSP stimulates repair of oxidative DNA lesions.
USP7 is involved in the cellular stress response by regulating Mdm2 and p53 protein levels following severe DNA damage. In addition to this, USP7 may also play a role in chromatin remodelling by direct deubiquitylation of histones, as well as indirectly by regulating the cellular levels of E3 ubiqui...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
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Oxford University Press
2011
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| _version_ | 1826265491334758400 |
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| author | Khoronenkova, S Dianova, I Parsons, J Dianov, G |
| author_facet | Khoronenkova, S Dianova, I Parsons, J Dianov, G |
| author_sort | Khoronenkova, S |
| collection | OXFORD |
| description | USP7 is involved in the cellular stress response by regulating Mdm2 and p53 protein levels following severe DNA damage. In addition to this, USP7 may also play a role in chromatin remodelling by direct deubiquitylation of histones, as well as indirectly by regulating the cellular levels of E3 ubiquitin ligases involved in histone ubiquitylation. Here, we provide new evidence that USP7 modulated chromatin remodelling is important for base excision repair of oxidative lesions. We show that transient USP7 siRNA knockdown did not change the levels or activity of base excision repair enzymes, but significantly reduced chromatin DNA accessibility and consequently the rate of repair of oxidative lesions. |
| first_indexed | 2024-03-06T20:24:31Z |
| format | Journal article |
| id | oxford-uuid:2ef3698e-aad5-463c-a796-e0e8a6aae868 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:24:31Z |
| publishDate | 2011 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | oxford-uuid:2ef3698e-aad5-463c-a796-e0e8a6aae8682022-03-26T12:52:09ZUSP7/HAUSP stimulates repair of oxidative DNA lesions.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2ef3698e-aad5-463c-a796-e0e8a6aae868EnglishSymplectic Elements at OxfordOxford University Press2011Khoronenkova, SDianova, IParsons, JDianov, GUSP7 is involved in the cellular stress response by regulating Mdm2 and p53 protein levels following severe DNA damage. In addition to this, USP7 may also play a role in chromatin remodelling by direct deubiquitylation of histones, as well as indirectly by regulating the cellular levels of E3 ubiquitin ligases involved in histone ubiquitylation. Here, we provide new evidence that USP7 modulated chromatin remodelling is important for base excision repair of oxidative lesions. We show that transient USP7 siRNA knockdown did not change the levels or activity of base excision repair enzymes, but significantly reduced chromatin DNA accessibility and consequently the rate of repair of oxidative lesions. |
| spellingShingle | Khoronenkova, S Dianova, I Parsons, J Dianov, G USP7/HAUSP stimulates repair of oxidative DNA lesions. |
| title | USP7/HAUSP stimulates repair of oxidative DNA lesions. |
| title_full | USP7/HAUSP stimulates repair of oxidative DNA lesions. |
| title_fullStr | USP7/HAUSP stimulates repair of oxidative DNA lesions. |
| title_full_unstemmed | USP7/HAUSP stimulates repair of oxidative DNA lesions. |
| title_short | USP7/HAUSP stimulates repair of oxidative DNA lesions. |
| title_sort | usp7 hausp stimulates repair of oxidative dna lesions |
| work_keys_str_mv | AT khoronenkovas usp7hauspstimulatesrepairofoxidativednalesions AT dianovai usp7hauspstimulatesrepairofoxidativednalesions AT parsonsj usp7hauspstimulatesrepairofoxidativednalesions AT dianovg usp7hauspstimulatesrepairofoxidativednalesions |