Molecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand.
Leptospirosis and scrub typhus are major causes of acute febrile illness in rural Asia, where co-infection is reported to occur based on serologic evidence. We re-examined whether co-infection occurs by using a molecular approach. A duplex real-time polymerase chain reaction was developed that targe...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2013
|
| _version_ | 1826265608037072896 |
|---|---|
| author | Sonthayanon, P Chierakul, W Wuthiekanun, V Limmathurotsakul, D Amornchai, P Smythe, L Day, N Peacock, S |
| author_facet | Sonthayanon, P Chierakul, W Wuthiekanun, V Limmathurotsakul, D Amornchai, P Smythe, L Day, N Peacock, S |
| author_sort | Sonthayanon, P |
| collection | OXFORD |
| description | Leptospirosis and scrub typhus are major causes of acute febrile illness in rural Asia, where co-infection is reported to occur based on serologic evidence. We re-examined whether co-infection occurs by using a molecular approach. A duplex real-time polymerase chain reaction was developed that targeted a specific 16S ribosomal RNA gene of pathogenic Leptospira spp. and Orientia tsutsugamushi. Of 82 patients with an acute febrile illness who had dual infection on the basis of serologic tests, 5 (6%) had polymerase chain reaction results positive for both pathogens. We conclude that dual infection occurs, but that serologic tests may overestimate the frequency of co-infections. |
| first_indexed | 2024-03-06T20:26:20Z |
| format | Journal article |
| id | oxford-uuid:2f83f7d5-8955-4493-b80f-3847b51021d9 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:26:20Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:2f83f7d5-8955-4493-b80f-3847b51021d92022-03-26T12:55:53ZMolecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2f83f7d5-8955-4493-b80f-3847b51021d9EnglishSymplectic Elements at Oxford2013Sonthayanon, PChierakul, WWuthiekanun, VLimmathurotsakul, DAmornchai, PSmythe, LDay, NPeacock, SLeptospirosis and scrub typhus are major causes of acute febrile illness in rural Asia, where co-infection is reported to occur based on serologic evidence. We re-examined whether co-infection occurs by using a molecular approach. A duplex real-time polymerase chain reaction was developed that targeted a specific 16S ribosomal RNA gene of pathogenic Leptospira spp. and Orientia tsutsugamushi. Of 82 patients with an acute febrile illness who had dual infection on the basis of serologic tests, 5 (6%) had polymerase chain reaction results positive for both pathogens. We conclude that dual infection occurs, but that serologic tests may overestimate the frequency of co-infections. |
| spellingShingle | Sonthayanon, P Chierakul, W Wuthiekanun, V Limmathurotsakul, D Amornchai, P Smythe, L Day, N Peacock, S Molecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand. |
| title | Molecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand. |
| title_full | Molecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand. |
| title_fullStr | Molecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand. |
| title_full_unstemmed | Molecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand. |
| title_short | Molecular confirmation of co-infection by pathogenic Leptospira spp. and Orientia tsutsugamushi in patients with acute febrile illness in Thailand. |
| title_sort | molecular confirmation of co infection by pathogenic leptospira spp and orientia tsutsugamushi in patients with acute febrile illness in thailand |
| work_keys_str_mv | AT sonthayanonp molecularconfirmationofcoinfectionbypathogenicleptospirasppandorientiatsutsugamushiinpatientswithacutefebrileillnessinthailand AT chierakulw molecularconfirmationofcoinfectionbypathogenicleptospirasppandorientiatsutsugamushiinpatientswithacutefebrileillnessinthailand AT wuthiekanunv molecularconfirmationofcoinfectionbypathogenicleptospirasppandorientiatsutsugamushiinpatientswithacutefebrileillnessinthailand AT limmathurotsakuld molecularconfirmationofcoinfectionbypathogenicleptospirasppandorientiatsutsugamushiinpatientswithacutefebrileillnessinthailand AT amornchaip molecularconfirmationofcoinfectionbypathogenicleptospirasppandorientiatsutsugamushiinpatientswithacutefebrileillnessinthailand AT smythel molecularconfirmationofcoinfectionbypathogenicleptospirasppandorientiatsutsugamushiinpatientswithacutefebrileillnessinthailand AT dayn molecularconfirmationofcoinfectionbypathogenicleptospirasppandorientiatsutsugamushiinpatientswithacutefebrileillnessinthailand AT peacocks molecularconfirmationofcoinfectionbypathogenicleptospirasppandorientiatsutsugamushiinpatientswithacutefebrileillnessinthailand |