Twelve ways to confirm targets of activity-based probes in plants
Activity-based probes are powerful tools to interrogate the functional proteome. Their covalent and often irreversible labeling of proteins facilitates the purification, identification and quantification of labeled proteins. However, the detection of labeled proteins often requires a confirmation, e...
| Những tác giả chính: | , |
|---|---|
| Định dạng: | Journal article |
| Được phát hành: |
Elsevier
2016
|
| _version_ | 1826265629687021568 |
|---|---|
| author | Kovács, J van der Hoorn, R |
| author_facet | Kovács, J van der Hoorn, R |
| author_sort | Kovács, J |
| collection | OXFORD |
| description | Activity-based probes are powerful tools to interrogate the functional proteome. Their covalent and often irreversible labeling of proteins facilitates the purification, identification and quantification of labeled proteins. However, the detection of labeled proteins often requires a confirmation, especially when unexpected proteins are identified, or to unravel fluorescent activity profiles. Here, we review twelve approaches towards target confirmation, grouped in approaches by direct target detection, target expression or target depletion. We discuss their proper use and limitations and illustrate these approaches with examples from plant science. |
| first_indexed | 2024-03-06T20:26:40Z |
| format | Journal article |
| id | oxford-uuid:2f9fc1b2-502d-4ad6-a1e7-57229d0fb37d |
| institution | University of Oxford |
| last_indexed | 2024-03-06T20:26:40Z |
| publishDate | 2016 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:2f9fc1b2-502d-4ad6-a1e7-57229d0fb37d2022-03-26T12:56:26ZTwelve ways to confirm targets of activity-based probes in plantsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:2f9fc1b2-502d-4ad6-a1e7-57229d0fb37dSymplectic Elements at OxfordElsevier2016Kovács, Jvan der Hoorn, RActivity-based probes are powerful tools to interrogate the functional proteome. Their covalent and often irreversible labeling of proteins facilitates the purification, identification and quantification of labeled proteins. However, the detection of labeled proteins often requires a confirmation, especially when unexpected proteins are identified, or to unravel fluorescent activity profiles. Here, we review twelve approaches towards target confirmation, grouped in approaches by direct target detection, target expression or target depletion. We discuss their proper use and limitations and illustrate these approaches with examples from plant science. |
| spellingShingle | Kovács, J van der Hoorn, R Twelve ways to confirm targets of activity-based probes in plants |
| title | Twelve ways to confirm targets of activity-based probes in plants |
| title_full | Twelve ways to confirm targets of activity-based probes in plants |
| title_fullStr | Twelve ways to confirm targets of activity-based probes in plants |
| title_full_unstemmed | Twelve ways to confirm targets of activity-based probes in plants |
| title_short | Twelve ways to confirm targets of activity-based probes in plants |
| title_sort | twelve ways to confirm targets of activity based probes in plants |
| work_keys_str_mv | AT kovacsj twelvewaystoconfirmtargetsofactivitybasedprobesinplants AT vanderhoornr twelvewaystoconfirmtargetsofactivitybasedprobesinplants |