High-throughput class I HLA genotyping using fluorescence resonance energy transfer (FRET) probes and sequence-specific primer-polymerase chain reaction (SSP-PCR).
We have developed a semi-automated HLA class I typing system utilising TET/TAMRA-labelled fluorescence resonance energy transfer (FRET) hydrolysis probes. The results from 87 individuals are in full concordance with serology and conventional gel-based systems. This assay replaces labour-intensive co...
Autori principali: | Gelsthorpe, A, Wells, R, Lowe, A, Tonks, S, Bodmer, J, Bodmer, W |
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Natura: | Journal article |
Lingua: | English |
Pubblicazione: |
1999
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