Phenotypic analysis of antigen-specific T lymphocytes.
Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tet...
| Автори: | , , , , , , , |
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| Формат: | Journal article |
| Мова: | English |
| Опубліковано: |
1996
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| _version_ | 1826265873622499328 |
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| author | Altman, J Moss, P Goulder, P Barouch, D McHeyzer-Williams, MG Bell, J McMichael, A Davis, M |
| author_facet | Altman, J Moss, P Goulder, P Barouch, D McHeyzer-Williams, MG Bell, J McMichael, A Davis, M |
| author_sort | Altman, J |
| collection | OXFORD |
| description | Identification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens. |
| first_indexed | 2024-03-06T20:30:26Z |
| format | Journal article |
| id | oxford-uuid:30da732b-b95c-43e6-a952-e8bb7f445aeb |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:30:26Z |
| publishDate | 1996 |
| record_format | dspace |
| spelling | oxford-uuid:30da732b-b95c-43e6-a952-e8bb7f445aeb2022-03-26T13:04:08ZPhenotypic analysis of antigen-specific T lymphocytes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:30da732b-b95c-43e6-a952-e8bb7f445aebEnglishSymplectic Elements at Oxford1996Altman, JMoss, PGoulder, PBarouch, DMcHeyzer-Williams, MGBell, JMcMichael, ADavis, MIdentification and characterization of antigen-specific T lymphocytes during the course of an immune response is tedious and indirect. To address this problem, the peptide-major histocompatability complex (MHC) ligand for a given population of T cells was multimerized to make soluble peptide-MHC tetramers. Tetramers of human lymphocyte antigen A2 that were complexed with two different human immunodeficiency virus (HIV)-derived peptides or with a peptide derived from influenza A matrix protein bound to peptide-specific cytotoxic T cells in vitro and to T cells from the blood of HIV-infected individuals. In general, tetramer binding correlated well with cytotoxicity assays. This approach should be useful in the analysis of T cells specific for infectious agents, tumors, and autoantigens. |
| spellingShingle | Altman, J Moss, P Goulder, P Barouch, D McHeyzer-Williams, MG Bell, J McMichael, A Davis, M Phenotypic analysis of antigen-specific T lymphocytes. |
| title | Phenotypic analysis of antigen-specific T lymphocytes. |
| title_full | Phenotypic analysis of antigen-specific T lymphocytes. |
| title_fullStr | Phenotypic analysis of antigen-specific T lymphocytes. |
| title_full_unstemmed | Phenotypic analysis of antigen-specific T lymphocytes. |
| title_short | Phenotypic analysis of antigen-specific T lymphocytes. |
| title_sort | phenotypic analysis of antigen specific t lymphocytes |
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