Plasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development.
Successful development of Plasmodium sexual stages is essential for parasite survival, but the genes involved are poorly understood. We 'knocked out' the male development gene-1 (mdv-1) locus in Plasmodium berghei and found it to be important in female gametocyte activation. Indirect immun...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2009
|
| _version_ | 1826266105892569088 |
|---|---|
| author | Lal, K Delves, M Bromley, E Wastling, J Tomley, F Sinden, R |
| author_facet | Lal, K Delves, M Bromley, E Wastling, J Tomley, F Sinden, R |
| author_sort | Lal, K |
| collection | OXFORD |
| description | Successful development of Plasmodium sexual stages is essential for parasite survival, but the genes involved are poorly understood. We 'knocked out' the male development gene-1 (mdv-1) locus in Plasmodium berghei and found it to be important in female gametocyte activation. Indirect immunofluorescence assays show MDV-1 has a punctate cytoplasmic distribution in gametocytes. After activation of both females and males, MDV-1 is more peripherally located but in males exclusively it becomes concentrated in a few large foci. In vitro ookinete conversion assays that test the ability of activated female gametocytes to develop into retort stage ookinetes, suggests a complicit role for MDV-1, with the knock-out parasite producing 86% reduction in ookinetes. The retort stage ookinete develops from the zygote by increasing growth of an apical protrusion and MDV-1 locates at the 'leading' extracellular apical pole of this protrusion. In the fully developed ookinete MDV-1 is localised to the posterior pole. In vivo, the knock-out parasites demonstrate a phenotype in which there is a 90% reduction of parasite transmission to oocysts in mosquitoes. |
| first_indexed | 2024-03-06T20:33:53Z |
| format | Journal article |
| id | oxford-uuid:31f82be8-4f05-4208-8195-f8960d76ad68 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:33:53Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:31f82be8-4f05-4208-8195-f8960d76ad682022-03-26T13:11:10ZPlasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:31f82be8-4f05-4208-8195-f8960d76ad68EnglishSymplectic Elements at Oxford2009Lal, KDelves, MBromley, EWastling, JTomley, FSinden, RSuccessful development of Plasmodium sexual stages is essential for parasite survival, but the genes involved are poorly understood. We 'knocked out' the male development gene-1 (mdv-1) locus in Plasmodium berghei and found it to be important in female gametocyte activation. Indirect immunofluorescence assays show MDV-1 has a punctate cytoplasmic distribution in gametocytes. After activation of both females and males, MDV-1 is more peripherally located but in males exclusively it becomes concentrated in a few large foci. In vitro ookinete conversion assays that test the ability of activated female gametocytes to develop into retort stage ookinetes, suggests a complicit role for MDV-1, with the knock-out parasite producing 86% reduction in ookinetes. The retort stage ookinete develops from the zygote by increasing growth of an apical protrusion and MDV-1 locates at the 'leading' extracellular apical pole of this protrusion. In the fully developed ookinete MDV-1 is localised to the posterior pole. In vivo, the knock-out parasites demonstrate a phenotype in which there is a 90% reduction of parasite transmission to oocysts in mosquitoes. |
| spellingShingle | Lal, K Delves, M Bromley, E Wastling, J Tomley, F Sinden, R Plasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development. |
| title | Plasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development. |
| title_full | Plasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development. |
| title_fullStr | Plasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development. |
| title_full_unstemmed | Plasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development. |
| title_short | Plasmodium male development gene-1 (mdv-1) is important for female sexual development and identifies a polarised plasma membrane during zygote development. |
| title_sort | plasmodium male development gene 1 mdv 1 is important for female sexual development and identifies a polarised plasma membrane during zygote development |
| work_keys_str_mv | AT lalk plasmodiummaledevelopmentgene1mdv1isimportantforfemalesexualdevelopmentandidentifiesapolarisedplasmamembraneduringzygotedevelopment AT delvesm plasmodiummaledevelopmentgene1mdv1isimportantforfemalesexualdevelopmentandidentifiesapolarisedplasmamembraneduringzygotedevelopment AT bromleye plasmodiummaledevelopmentgene1mdv1isimportantforfemalesexualdevelopmentandidentifiesapolarisedplasmamembraneduringzygotedevelopment AT wastlingj plasmodiummaledevelopmentgene1mdv1isimportantforfemalesexualdevelopmentandidentifiesapolarisedplasmamembraneduringzygotedevelopment AT tomleyf plasmodiummaledevelopmentgene1mdv1isimportantforfemalesexualdevelopmentandidentifiesapolarisedplasmamembraneduringzygotedevelopment AT sindenr plasmodiummaledevelopmentgene1mdv1isimportantforfemalesexualdevelopmentandidentifiesapolarisedplasmamembraneduringzygotedevelopment |