DNA sequence recognition by an isopropyl substituted thiazole polyamide.
We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted thiazole polyamide, to DNA. In one fragment, which contains every tetranucleotide sequence, sub-micr...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2004
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| _version_ | 1826266192848879616 |
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| author | James, P Merkina, E Khalaf, A Suckling, C Waigh, R Brown, T Fox, K |
| author_facet | James, P Merkina, E Khalaf, A Suckling, C Waigh, R Brown, T Fox, K |
| author_sort | James, P |
| collection | OXFORD |
| description | We have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted thiazole polyamide, to DNA. In one fragment, which contains every tetranucleotide sequence, sub-micromolar concentrations of the ligand generate a single footprint at the sequence ACTAGT. This sequence preference is confirmed in melting experiments with fluorescently labelled oligonucleotides. Experiments with DNA fragments that contain variants of this sequence suggest that the ligand also binds, with slightly lower affinity, to sequences of the type XCYRGZ, where X is any base except C, and Z is any base except G. |
| first_indexed | 2024-03-06T20:35:11Z |
| format | Journal article |
| id | oxford-uuid:32658ba2-a053-4ec0-8a7a-1d1bcdd5897d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:35:11Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:32658ba2-a053-4ec0-8a7a-1d1bcdd5897d2022-03-26T13:13:44ZDNA sequence recognition by an isopropyl substituted thiazole polyamide.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:32658ba2-a053-4ec0-8a7a-1d1bcdd5897dEnglishSymplectic Elements at Oxford2004James, PMerkina, EKhalaf, ASuckling, CWaigh, RBrown, TFox, KWe have used DNA footprinting and fluorescence melting experiments to study the sequence-specific binding of a novel minor groove binding ligand (thiazotropsin A), containing an isopropyl substituted thiazole polyamide, to DNA. In one fragment, which contains every tetranucleotide sequence, sub-micromolar concentrations of the ligand generate a single footprint at the sequence ACTAGT. This sequence preference is confirmed in melting experiments with fluorescently labelled oligonucleotides. Experiments with DNA fragments that contain variants of this sequence suggest that the ligand also binds, with slightly lower affinity, to sequences of the type XCYRGZ, where X is any base except C, and Z is any base except G. |
| spellingShingle | James, P Merkina, E Khalaf, A Suckling, C Waigh, R Brown, T Fox, K DNA sequence recognition by an isopropyl substituted thiazole polyamide. |
| title | DNA sequence recognition by an isopropyl substituted thiazole polyamide. |
| title_full | DNA sequence recognition by an isopropyl substituted thiazole polyamide. |
| title_fullStr | DNA sequence recognition by an isopropyl substituted thiazole polyamide. |
| title_full_unstemmed | DNA sequence recognition by an isopropyl substituted thiazole polyamide. |
| title_short | DNA sequence recognition by an isopropyl substituted thiazole polyamide. |
| title_sort | dna sequence recognition by an isopropyl substituted thiazole polyamide |
| work_keys_str_mv | AT jamesp dnasequencerecognitionbyanisopropylsubstitutedthiazolepolyamide AT merkinae dnasequencerecognitionbyanisopropylsubstitutedthiazolepolyamide AT khalafa dnasequencerecognitionbyanisopropylsubstitutedthiazolepolyamide AT sucklingc dnasequencerecognitionbyanisopropylsubstitutedthiazolepolyamide AT waighr dnasequencerecognitionbyanisopropylsubstitutedthiazolepolyamide AT brownt dnasequencerecognitionbyanisopropylsubstitutedthiazolepolyamide AT foxk dnasequencerecognitionbyanisopropylsubstitutedthiazolepolyamide |