High-resolution patterned cellular constructs by droplet-based 3D printing.
Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here w...
| मुख्य लेखकों: | , , , , , , , , , |
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| स्वरूप: | Journal article |
| भाषा: | English |
| प्रकाशित: |
Nature Publishing Group
2017
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| _version_ | 1826266204372729856 |
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| author | Graham, AD Olof, SN Burke, MJ Armstrong, JPK Mikhailova, EA Nicholson, JG Box, SJ Szele, FG Perriman, AW Bayley, JHP |
| author_facet | Graham, AD Olof, SN Burke, MJ Armstrong, JPK Mikhailova, EA Nicholson, JG Box, SJ Szele, FG Perriman, AW Bayley, JHP |
| author_sort | Graham, AD |
| collection | OXFORD |
| description | Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here we present a low-cost process that employs 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs in oil, which were reproducibly transferred to culture medium. Human embryonic kidney (HEK) cells and ovine mesenchymal stem cells (oMSCs) were printed at tissue-relevant densities (107 cells mL-1) and a high droplet resolution of 1 nL. High-resolution 3D geometries were printed with features of ≤200 μm; these included an arborised cell junction, a diagonal-plane junction and an osteochondral interface. The printed cells showed high viability (90% on average) and HEK cells within the printed structures were shown to proliferate under culture conditions. Significantly, a five-week tissue engineering study demonstrated that printed oMSCs could be differentiated down the chondrogenic lineage to generate cartilage-like structures containing type II collagen. |
| first_indexed | 2024-03-06T20:35:21Z |
| format | Journal article |
| id | oxford-uuid:327127d4-01c6-4103-aed5-c40cb646e30e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:35:21Z |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | dspace |
| spelling | oxford-uuid:327127d4-01c6-4103-aed5-c40cb646e30e2022-03-26T13:14:10ZHigh-resolution patterned cellular constructs by droplet-based 3D printing.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:327127d4-01c6-4103-aed5-c40cb646e30eEnglishSymplectic Elements at OxfordNature Publishing Group2017Graham, ADOlof, SNBurke, MJArmstrong, JPKMikhailova, EANicholson, JGBox, SJSzele, FGPerriman, AWBayley, JHPBioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here we present a low-cost process that employs 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs in oil, which were reproducibly transferred to culture medium. Human embryonic kidney (HEK) cells and ovine mesenchymal stem cells (oMSCs) were printed at tissue-relevant densities (107 cells mL-1) and a high droplet resolution of 1 nL. High-resolution 3D geometries were printed with features of ≤200 μm; these included an arborised cell junction, a diagonal-plane junction and an osteochondral interface. The printed cells showed high viability (90% on average) and HEK cells within the printed structures were shown to proliferate under culture conditions. Significantly, a five-week tissue engineering study demonstrated that printed oMSCs could be differentiated down the chondrogenic lineage to generate cartilage-like structures containing type II collagen. |
| spellingShingle | Graham, AD Olof, SN Burke, MJ Armstrong, JPK Mikhailova, EA Nicholson, JG Box, SJ Szele, FG Perriman, AW Bayley, JHP High-resolution patterned cellular constructs by droplet-based 3D printing. |
| title | High-resolution patterned cellular constructs by droplet-based 3D printing. |
| title_full | High-resolution patterned cellular constructs by droplet-based 3D printing. |
| title_fullStr | High-resolution patterned cellular constructs by droplet-based 3D printing. |
| title_full_unstemmed | High-resolution patterned cellular constructs by droplet-based 3D printing. |
| title_short | High-resolution patterned cellular constructs by droplet-based 3D printing. |
| title_sort | high resolution patterned cellular constructs by droplet based 3d printing |
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