Okazaki fragment processing during eukaryotic DNA replication
<p style="text-align:justify;"> Okazaki fragments containing an RNA primer are synthesised on the lagging strand of the eukaryotic replication fork. Major advances have recently been made in our understanding of the complex process of Okazaki fragment processing to form high molecul...
| Main Author: | |
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| Format: | Journal article |
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Yodosha
1998
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| _version_ | 1826266215869317120 |
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| author | Cox, L |
| author_facet | Cox, L |
| author_sort | Cox, L |
| collection | OXFORD |
| description | <p style="text-align:justify;"> Okazaki fragments containing an RNA primer are synthesised on the lagging strand of the eukaryotic replication fork. Major advances have recently been made in our understanding of the complex process of Okazaki fragment processing to form high molecular weight daughter DNA, identifying distinct stages. Firstly, primase synthesises an RNA primer which is rapidly extended into DNA by bound DNA polymerase α. DNA pol ε is then thought to synthesise the remainder of the fragment (50-200 nucleotides). The RNA primer is removed almost intact by RNaseH1, leaving a single ribonucleotide that is cleaved by the FEN-1 nuclease. DNA originally synthesised by pol α is replaced by DNA pol ε in co-operation with FEN-1. Finally, DNA ligase 1 seals the resulting nick by forming a phosphodiester bond between adjacent DNA nucleotides. A model whereby 2 these complex processes are modulated by DNA context and regulatory protein-protein interactions is presented. </p> |
| first_indexed | 2024-03-06T20:35:33Z |
| format | Journal article |
| id | oxford-uuid:328258d8-33b9-4815-9797-4169f2f771dd |
| institution | University of Oxford |
| last_indexed | 2024-03-06T20:35:33Z |
| publishDate | 1998 |
| publisher | Yodosha |
| record_format | dspace |
| spelling | oxford-uuid:328258d8-33b9-4815-9797-4169f2f771dd2022-03-26T13:14:27ZOkazaki fragment processing during eukaryotic DNA replicationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:328258d8-33b9-4815-9797-4169f2f771ddSymplectic Elements at OxfordYodosha1998Cox, L <p style="text-align:justify;"> Okazaki fragments containing an RNA primer are synthesised on the lagging strand of the eukaryotic replication fork. Major advances have recently been made in our understanding of the complex process of Okazaki fragment processing to form high molecular weight daughter DNA, identifying distinct stages. Firstly, primase synthesises an RNA primer which is rapidly extended into DNA by bound DNA polymerase α. DNA pol ε is then thought to synthesise the remainder of the fragment (50-200 nucleotides). The RNA primer is removed almost intact by RNaseH1, leaving a single ribonucleotide that is cleaved by the FEN-1 nuclease. DNA originally synthesised by pol α is replaced by DNA pol ε in co-operation with FEN-1. Finally, DNA ligase 1 seals the resulting nick by forming a phosphodiester bond between adjacent DNA nucleotides. A model whereby 2 these complex processes are modulated by DNA context and regulatory protein-protein interactions is presented. </p> |
| spellingShingle | Cox, L Okazaki fragment processing during eukaryotic DNA replication |
| title | Okazaki fragment processing during eukaryotic DNA replication |
| title_full | Okazaki fragment processing during eukaryotic DNA replication |
| title_fullStr | Okazaki fragment processing during eukaryotic DNA replication |
| title_full_unstemmed | Okazaki fragment processing during eukaryotic DNA replication |
| title_short | Okazaki fragment processing during eukaryotic DNA replication |
| title_sort | okazaki fragment processing during eukaryotic dna replication |
| work_keys_str_mv | AT coxl okazakifragmentprocessingduringeukaryoticdnareplication |