Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system.
Advances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 microm liquid chromatography (UPLC ACQUITY) and LTQ-Orbitrap mass spectrometry systems provides many theoretica...
| मुख्य लेखकों: | , , , , , , |
|---|---|
| स्वरूप: | Journal article |
| भाषा: | English |
| प्रकाशित: |
2008
|
| _version_ | 1826266218166747136 |
|---|---|
| author | Dunn, W Broadhurst, D Brown, M Baker, P Redman, C Kenny, L Kell, D |
| author_facet | Dunn, W Broadhurst, D Brown, M Baker, P Redman, C Kenny, L Kell, D |
| author_sort | Dunn, W |
| collection | OXFORD |
| description | Advances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 microm liquid chromatography (UPLC ACQUITY) and LTQ-Orbitrap mass spectrometry systems provides many theoretical advantages. The applicability of the interfaced systems was investigated using a simple 11-component metabolite mix and a complex mammalian biofluid, serum. Metabolites were detected in the metabolite mix with signals that were linear with their concentration over 2.5-3.5 orders of magnitude, with correlation coefficients greater than 0.993 and limits of detection less than 1 micromol L(-1). Reproducibility of retention time (RSD<3%) and chromatographic peak area (RSD<15%) and a high mass accuracy (<2 ppm) were observed for 14 QC serum samples interdispersed with other serum samples, analysed over a period of 40 h. The evaluation of a single deconvolution software package (XCMS) was performed and showed that two parameters (snthresh and bw) provided significant changes to the number of peaks detected and the peak area reproducibility for the dataset used. The data were used to indicate possible biomarkers of pre-eclampsia and showed both the instruments and XCMS to be applicable to the reproducible and valid detection of disease biomarkers present in serum. |
| first_indexed | 2024-03-06T20:35:34Z |
| format | Journal article |
| id | oxford-uuid:3283a071-f0b6-49fe-aa8d-a14855347c82 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:35:34Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | oxford-uuid:3283a071-f0b6-49fe-aa8d-a14855347c822022-03-26T13:14:31ZMetabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3283a071-f0b6-49fe-aa8d-a14855347c82EnglishSymplectic Elements at Oxford2008Dunn, WBroadhurst, DBrown, MBaker, PRedman, CKenny, LKell, DAdvances in analytical instrumentation can provide significant advantages to the volume and quality of biological knowledge acquired in metabolomic investigations. The interfacing of sub-2 microm liquid chromatography (UPLC ACQUITY) and LTQ-Orbitrap mass spectrometry systems provides many theoretical advantages. The applicability of the interfaced systems was investigated using a simple 11-component metabolite mix and a complex mammalian biofluid, serum. Metabolites were detected in the metabolite mix with signals that were linear with their concentration over 2.5-3.5 orders of magnitude, with correlation coefficients greater than 0.993 and limits of detection less than 1 micromol L(-1). Reproducibility of retention time (RSD<3%) and chromatographic peak area (RSD<15%) and a high mass accuracy (<2 ppm) were observed for 14 QC serum samples interdispersed with other serum samples, analysed over a period of 40 h. The evaluation of a single deconvolution software package (XCMS) was performed and showed that two parameters (snthresh and bw) provided significant changes to the number of peaks detected and the peak area reproducibility for the dataset used. The data were used to indicate possible biomarkers of pre-eclampsia and showed both the instruments and XCMS to be applicable to the reproducible and valid detection of disease biomarkers present in serum. |
| spellingShingle | Dunn, W Broadhurst, D Brown, M Baker, P Redman, C Kenny, L Kell, D Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. |
| title | Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. |
| title_full | Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. |
| title_fullStr | Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. |
| title_full_unstemmed | Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. |
| title_short | Metabolic profiling of serum using Ultra Performance Liquid Chromatography and the LTQ-Orbitrap mass spectrometry system. |
| title_sort | metabolic profiling of serum using ultra performance liquid chromatography and the ltq orbitrap mass spectrometry system |
| work_keys_str_mv | AT dunnw metabolicprofilingofserumusingultraperformanceliquidchromatographyandtheltqorbitrapmassspectrometrysystem AT broadhurstd metabolicprofilingofserumusingultraperformanceliquidchromatographyandtheltqorbitrapmassspectrometrysystem AT brownm metabolicprofilingofserumusingultraperformanceliquidchromatographyandtheltqorbitrapmassspectrometrysystem AT bakerp metabolicprofilingofserumusingultraperformanceliquidchromatographyandtheltqorbitrapmassspectrometrysystem AT redmanc metabolicprofilingofserumusingultraperformanceliquidchromatographyandtheltqorbitrapmassspectrometrysystem AT kennyl metabolicprofilingofserumusingultraperformanceliquidchromatographyandtheltqorbitrapmassspectrometrysystem AT kelld metabolicprofilingofserumusingultraperformanceliquidchromatographyandtheltqorbitrapmassspectrometrysystem |