The design of microfluidic affinity chromatography systems for the separation of bioanalytes.
The analytical (numerical) design of planar microfluidic affinity chromatography devices, which consist of multiple separation lanes and multiple, different surface-immobilised receptor patterns in each lane, is described. The model is based on the analytical solution of the transport-reaction equat...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2012
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| _version_ | 1797061809204625408 |
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| author | Friedrich, D Please, C Melvin, T |
| author_facet | Friedrich, D Please, C Melvin, T |
| author_sort | Friedrich, D |
| collection | OXFORD |
| description | The analytical (numerical) design of planar microfluidic affinity chromatography devices, which consist of multiple separation lanes and multiple, different surface-immobilised receptor patterns in each lane, is described. The model is based on the analytical solution of the transport-reaction equations in microfluidic systems of low Gratz number and for injection of small analyte plugs. The results reveal a simple approach for the design of microfluidic affinity chromatography devices tailored to the separation of bioanalytes, where receptors with high binding affinity are available. These devices have been designed for bioanalytical applications in mind, most notably for the proteomics field; the results are illustrated with an example using β-Amyloid binding peptides. |
| first_indexed | 2024-03-06T20:36:31Z |
| format | Journal article |
| id | oxford-uuid:32d2061e-5cf8-4ff5-aed4-78cb5d935991 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:36:31Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:32d2061e-5cf8-4ff5-aed4-78cb5d9359912022-03-26T13:16:26ZThe design of microfluidic affinity chromatography systems for the separation of bioanalytes.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:32d2061e-5cf8-4ff5-aed4-78cb5d935991EnglishSymplectic Elements at Oxford2012Friedrich, DPlease, CMelvin, TThe analytical (numerical) design of planar microfluidic affinity chromatography devices, which consist of multiple separation lanes and multiple, different surface-immobilised receptor patterns in each lane, is described. The model is based on the analytical solution of the transport-reaction equations in microfluidic systems of low Gratz number and for injection of small analyte plugs. The results reveal a simple approach for the design of microfluidic affinity chromatography devices tailored to the separation of bioanalytes, where receptors with high binding affinity are available. These devices have been designed for bioanalytical applications in mind, most notably for the proteomics field; the results are illustrated with an example using β-Amyloid binding peptides. |
| spellingShingle | Friedrich, D Please, C Melvin, T The design of microfluidic affinity chromatography systems for the separation of bioanalytes. |
| title | The design of microfluidic affinity chromatography systems for the separation of bioanalytes. |
| title_full | The design of microfluidic affinity chromatography systems for the separation of bioanalytes. |
| title_fullStr | The design of microfluidic affinity chromatography systems for the separation of bioanalytes. |
| title_full_unstemmed | The design of microfluidic affinity chromatography systems for the separation of bioanalytes. |
| title_short | The design of microfluidic affinity chromatography systems for the separation of bioanalytes. |
| title_sort | design of microfluidic affinity chromatography systems for the separation of bioanalytes |
| work_keys_str_mv | AT friedrichd thedesignofmicrofluidicaffinitychromatographysystemsfortheseparationofbioanalytes AT pleasec thedesignofmicrofluidicaffinitychromatographysystemsfortheseparationofbioanalytes AT melvint thedesignofmicrofluidicaffinitychromatographysystemsfortheseparationofbioanalytes AT friedrichd designofmicrofluidicaffinitychromatographysystemsfortheseparationofbioanalytes AT pleasec designofmicrofluidicaffinitychromatographysystemsfortheseparationofbioanalytes AT melvint designofmicrofluidicaffinitychromatographysystemsfortheseparationofbioanalytes |