Wavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupils

In adaptive optical microscopy of thick biological tissue, strong scattering and aberrations can change the effective pupil shape by rendering some Shack-Hartmann spots unusable. The change of pupil shape leads to a change of wavefront reconstruction or control matrix that should be updated accordin...

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Autores principales: Dong, B, Booth, M
Formato: Journal article
Publicado: Optical Society of America 2018
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author Dong, B
Booth, M
author_facet Dong, B
Booth, M
author_sort Dong, B
collection OXFORD
description In adaptive optical microscopy of thick biological tissue, strong scattering and aberrations can change the effective pupil shape by rendering some Shack-Hartmann spots unusable. The change of pupil shape leads to a change of wavefront reconstruction or control matrix that should be updated accordingly. Modified slope and modal wavefront control methods based on measurements of a Shack-Hartmann wavefront sensor are proposed to accommodate an arbitrarily shaped pupil. Furthermore, we present partial wavefront control methods that remove specific aberration modes like tip, tilt and defocus from the control loop. The proposed control methods were investigated and compared by simulation using experimentally obtained aberration data. The performance was then tested experimentally through closed-loop aberration corrections using an obscured pupil.
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spelling oxford-uuid:33261dae-217d-4192-be39-9e0bf069122a2022-03-26T13:18:36ZWavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupilsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:33261dae-217d-4192-be39-9e0bf069122aSymplectic Elements at OxfordOptical Society of America2018Dong, BBooth, MIn adaptive optical microscopy of thick biological tissue, strong scattering and aberrations can change the effective pupil shape by rendering some Shack-Hartmann spots unusable. The change of pupil shape leads to a change of wavefront reconstruction or control matrix that should be updated accordingly. Modified slope and modal wavefront control methods based on measurements of a Shack-Hartmann wavefront sensor are proposed to accommodate an arbitrarily shaped pupil. Furthermore, we present partial wavefront control methods that remove specific aberration modes like tip, tilt and defocus from the control loop. The proposed control methods were investigated and compared by simulation using experimentally obtained aberration data. The performance was then tested experimentally through closed-loop aberration corrections using an obscured pupil.
spellingShingle Dong, B
Booth, M
Wavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupils
title Wavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupils
title_full Wavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupils
title_fullStr Wavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupils
title_full_unstemmed Wavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupils
title_short Wavefront control in adaptive microscopy using Shack-Hartmann sensors with arbitrarily shaped pupils
title_sort wavefront control in adaptive microscopy using shack hartmann sensors with arbitrarily shaped pupils
work_keys_str_mv AT dongb wavefrontcontrolinadaptivemicroscopyusingshackhartmannsensorswitharbitrarilyshapedpupils
AT boothm wavefrontcontrolinadaptivemicroscopyusingshackhartmannsensorswitharbitrarilyshapedpupils