Hepatic glucose output in humans measured with labeled glucose to reduce negative errors.
Steele and others have suggested that minimizing changes in glucose specific activity when estimating hepatic glucose output (HGO) during glucose infusions could reduce non-steady-state errors. This approach was assessed in nondiabetic and type II diabetic subjects during constant low dose [27 mumol...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1989
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| _version_ | 1797061900239896576 |
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| author | Levy, J Brown, G Matthews, DR Turner, R |
| author_facet | Levy, J Brown, G Matthews, DR Turner, R |
| author_sort | Levy, J |
| collection | OXFORD |
| description | Steele and others have suggested that minimizing changes in glucose specific activity when estimating hepatic glucose output (HGO) during glucose infusions could reduce non-steady-state errors. This approach was assessed in nondiabetic and type II diabetic subjects during constant low dose [27 mumol.kg ideal body wt (IBW)-1.min-1] glucose infusion followed by a 12 mmol/l hyperglycemic clamp. Eight subjects had paired tests with and without labeled infusions. Labeled infusion was used to compare HGO in 11 nondiabetic and 15 diabetic subjects. Whereas unlabeled infusions produced negative values for endogenous glucose output, labeled infusions largely eliminated this error and reduced the dependence of the Steele model on the pool fraction in the paired tests. By use of labeled infusions, 11 nondiabetic subjects suppressed HGO from 10.2 +/- 0.6 (SE) fasting to 0.8 +/- 0.9 mumol.kg IBW-1.min-1 after 90 min of glucose infusion and to -1.9 +/- 0.5 mumol.kg IBW-1.min-1 after 90 min of a 12 mmol/l glucose clamp, but 15 diabetic subjects suppressed only partially from 13.0 +/- 0.9 fasting to 5.7 +/- 1.2 at the end of the glucose infusion and 5.6 +/- 1.0 mumol.kg IBW-1.min-1 in the clamp (P = 0.02, 0.002, and less than 0.001, respectively). |
| first_indexed | 2024-03-06T20:37:47Z |
| format | Journal article |
| id | oxford-uuid:3333f471-d4c2-4081-a80b-fbc3640fb62d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:37:47Z |
| publishDate | 1989 |
| record_format | dspace |
| spelling | oxford-uuid:3333f471-d4c2-4081-a80b-fbc3640fb62d2022-03-26T13:18:57ZHepatic glucose output in humans measured with labeled glucose to reduce negative errors.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:3333f471-d4c2-4081-a80b-fbc3640fb62dEnglishSymplectic Elements at Oxford1989Levy, JBrown, GMatthews, DRTurner, RSteele and others have suggested that minimizing changes in glucose specific activity when estimating hepatic glucose output (HGO) during glucose infusions could reduce non-steady-state errors. This approach was assessed in nondiabetic and type II diabetic subjects during constant low dose [27 mumol.kg ideal body wt (IBW)-1.min-1] glucose infusion followed by a 12 mmol/l hyperglycemic clamp. Eight subjects had paired tests with and without labeled infusions. Labeled infusion was used to compare HGO in 11 nondiabetic and 15 diabetic subjects. Whereas unlabeled infusions produced negative values for endogenous glucose output, labeled infusions largely eliminated this error and reduced the dependence of the Steele model on the pool fraction in the paired tests. By use of labeled infusions, 11 nondiabetic subjects suppressed HGO from 10.2 +/- 0.6 (SE) fasting to 0.8 +/- 0.9 mumol.kg IBW-1.min-1 after 90 min of glucose infusion and to -1.9 +/- 0.5 mumol.kg IBW-1.min-1 after 90 min of a 12 mmol/l glucose clamp, but 15 diabetic subjects suppressed only partially from 13.0 +/- 0.9 fasting to 5.7 +/- 1.2 at the end of the glucose infusion and 5.6 +/- 1.0 mumol.kg IBW-1.min-1 in the clamp (P = 0.02, 0.002, and less than 0.001, respectively). |
| spellingShingle | Levy, J Brown, G Matthews, DR Turner, R Hepatic glucose output in humans measured with labeled glucose to reduce negative errors. |
| title | Hepatic glucose output in humans measured with labeled glucose to reduce negative errors. |
| title_full | Hepatic glucose output in humans measured with labeled glucose to reduce negative errors. |
| title_fullStr | Hepatic glucose output in humans measured with labeled glucose to reduce negative errors. |
| title_full_unstemmed | Hepatic glucose output in humans measured with labeled glucose to reduce negative errors. |
| title_short | Hepatic glucose output in humans measured with labeled glucose to reduce negative errors. |
| title_sort | hepatic glucose output in humans measured with labeled glucose to reduce negative errors |
| work_keys_str_mv | AT levyj hepaticglucoseoutputinhumansmeasuredwithlabeledglucosetoreducenegativeerrors AT browng hepaticglucoseoutputinhumansmeasuredwithlabeledglucosetoreducenegativeerrors AT matthewsdr hepaticglucoseoutputinhumansmeasuredwithlabeledglucosetoreducenegativeerrors AT turnerr hepaticglucoseoutputinhumansmeasuredwithlabeledglucosetoreducenegativeerrors |