The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci
Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. Both occurred in the otherwise contrasting histories of the antibiotic-resistant S. pneumoniae lineages PMEN3 and PMEN9. A si...
| Main Authors: | , , , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal article |
| Language: | English |
| Published: |
eLife Sciences Publications
2021
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| _version_ | 1826266441983197184 |
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| author | D'Aeth, JC van der Linden, MP McGee, L de Lencastre, H Turner, P Song, J-H Lo, SW Gladstone, RA Sá-Leão, R Ko, KS Hanage, WP Breiman, RF Beall, B Bentley, SD Croucher, NJ |
| author2 | GPS Consortium |
| author_facet | GPS Consortium D'Aeth, JC van der Linden, MP McGee, L de Lencastre, H Turner, P Song, J-H Lo, SW Gladstone, RA Sá-Leão, R Ko, KS Hanage, WP Breiman, RF Beall, B Bentley, SD Croucher, NJ |
| author_sort | D'Aeth, JC |
| collection | OXFORD |
| description | Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. Both occurred in the otherwise contrasting histories of the antibiotic-resistant S. pneumoniae lineages PMEN3 and PMEN9. A single PMEN3 clade spread globally, evading vaccine-induced immunity through frequent serotype switching, whereas locally circulating PMEN9 clades independently gained resistance. Both lineages repeatedly integrated Tn916-type and Tn1207.1-type elements, conferring tetracycline and macrolide resistance, respectively, through homologous recombination importing sequences originating in other species. A species-wide dataset found over 100 instances of such interspecific acquisitions of resistance cassettes and flanking homologous arms. Phylodynamic analysis of the most commonly sampled Tn1207.1-type insertion in PMEN9, originating from a commensal and disrupting a competence gene, suggested its expansion across Germany was driven by a high ratio of macrolide-to-β-lactam consumption. Hence, selection from antibiotic consumption was sufficient for these atypically large recombinations to overcome species boundaries across the pneumococcal chromosome. |
| first_indexed | 2024-03-06T20:39:01Z |
| format | Journal article |
| id | oxford-uuid:33a1593d-abd0-41fb-a932-d434e91a746e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:39:01Z |
| publishDate | 2021 |
| publisher | eLife Sciences Publications |
| record_format | dspace |
| spelling | oxford-uuid:33a1593d-abd0-41fb-a932-d434e91a746e2022-03-26T13:21:17ZThe role of interspecies recombination in the evolution of antibiotic-resistant pneumococciJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:33a1593d-abd0-41fb-a932-d434e91a746eEnglishSymplectic ElementseLife Sciences Publications2021D'Aeth, JCvan der Linden, MPMcGee, Lde Lencastre, HTurner, PSong, J-HLo, SWGladstone, RASá-Leão, RKo, KSHanage, WPBreiman, RFBeall, BBentley, SDCroucher, NJGPS ConsortiumMultidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. Both occurred in the otherwise contrasting histories of the antibiotic-resistant S. pneumoniae lineages PMEN3 and PMEN9. A single PMEN3 clade spread globally, evading vaccine-induced immunity through frequent serotype switching, whereas locally circulating PMEN9 clades independently gained resistance. Both lineages repeatedly integrated Tn916-type and Tn1207.1-type elements, conferring tetracycline and macrolide resistance, respectively, through homologous recombination importing sequences originating in other species. A species-wide dataset found over 100 instances of such interspecific acquisitions of resistance cassettes and flanking homologous arms. Phylodynamic analysis of the most commonly sampled Tn1207.1-type insertion in PMEN9, originating from a commensal and disrupting a competence gene, suggested its expansion across Germany was driven by a high ratio of macrolide-to-β-lactam consumption. Hence, selection from antibiotic consumption was sufficient for these atypically large recombinations to overcome species boundaries across the pneumococcal chromosome. |
| spellingShingle | D'Aeth, JC van der Linden, MP McGee, L de Lencastre, H Turner, P Song, J-H Lo, SW Gladstone, RA Sá-Leão, R Ko, KS Hanage, WP Breiman, RF Beall, B Bentley, SD Croucher, NJ The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci |
| title | The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci |
| title_full | The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci |
| title_fullStr | The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci |
| title_full_unstemmed | The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci |
| title_short | The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci |
| title_sort | role of interspecies recombination in the evolution of antibiotic resistant pneumococci |
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