A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations.
A label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Up...
| Main Authors: | , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2012
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| _version_ | 1797062076912369664 |
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| author | Johnson, R Richardson, J Brown, T Bartlett, P |
| author_facet | Johnson, R Richardson, J Brown, T Bartlett, P |
| author_sort | Johnson, R |
| collection | OXFORD |
| description | A label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Upon application of a negative potential, the dsDNA denatures into its constituent strands, and the changes in the spectra of the reporter molecule are monitored. This method has been used to distinguish between a wild-type, 1653C/T single-point mutation and ΔF508 triplet deletion in the CFTR gene. The use of dsDNA-selective binding agents as reporter molecules in a discrimination assay removes the burden of synthetically modifying the target to be detected, while retaining flexibility in the choice of the reporter molecule. |
| first_indexed | 2024-03-06T20:40:23Z |
| format | Journal article |
| id | oxford-uuid:34184609-a12d-48e1-a1f1-bb58856714d7 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:40:23Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:34184609-a12d-48e1-a1f1-bb58856714d72022-03-26T13:23:48ZA label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:34184609-a12d-48e1-a1f1-bb58856714d7EnglishSymplectic Elements at Oxford2012Johnson, RRichardson, JBrown, TBartlett, PA label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Upon application of a negative potential, the dsDNA denatures into its constituent strands, and the changes in the spectra of the reporter molecule are monitored. This method has been used to distinguish between a wild-type, 1653C/T single-point mutation and ΔF508 triplet deletion in the CFTR gene. The use of dsDNA-selective binding agents as reporter molecules in a discrimination assay removes the burden of synthetically modifying the target to be detected, while retaining flexibility in the choice of the reporter molecule. |
| spellingShingle | Johnson, R Richardson, J Brown, T Bartlett, P A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations. |
| title | A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations. |
| title_full | A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations. |
| title_fullStr | A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations. |
| title_full_unstemmed | A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations. |
| title_short | A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations. |
| title_sort | label free electrochemical sers based assay for detection of dna hybridization and discrimination of mutations |
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