Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.

Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.

5-Azidomethyl dUTP and two 5-trans-cyclooctene dUTPs with different linkers between the TCO and the uracil base have been incorporated into DNA by primer extension, reverse-transcription and PCR amplification. For azidomethyl dUTP the PCR reaction was successful even when the modified dUTP was not s...

Ausführliche Beschreibung

Bibliographische Detailangaben
Hauptverfasser:	Ren, X, El-Sagheer, A, Brown, T
Format:	Journal article
Sprache:	English
Veröffentlicht:	Royal Society of Chemistry 2015

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