Plasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration.
An in vitro model of Plasmodium falciparum-infected red blood cell sequestration which uses C32 amelanotic melanoma cells as targets has been used to examine the binding capacity of infected red blood cells from subjects with naturally acquired P. falciparum infections of varying severity. The bindi...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1988
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| _version_ | 1797062159013773312 |
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| author | Marsh, K Marsh, V Brown, J Whittle, H Greenwood, B |
| author_facet | Marsh, K Marsh, V Brown, J Whittle, H Greenwood, B |
| author_sort | Marsh, K |
| collection | OXFORD |
| description | An in vitro model of Plasmodium falciparum-infected red blood cell sequestration which uses C32 amelanotic melanoma cells as targets has been used to examine the binding capacity of infected red blood cells from subjects with naturally acquired P. falciparum infections of varying severity. The binding of infected red blood cells (IRBCs) to melanoma cells was specific to cells containing mature parasites. Variations in target cell density and in conditions of growth had significant effects on binding. Binding was pH dependent, being maximum at a pH of 6.9. Using standardized conditions the binding capacity of individual isolates of P. falciparum could be measured with a high degree of reproducibility. Binding capacity of IRBCs from 51 subjects between the ages of 6 months and 15 years varied between 12 and 1254 IRBCs per 100 melanoma cells when RBC suspensions at a 1% parasitemia and 4% hematocrit were used. Variation in binding was not related to the level of peripheral parasitemia of the isolate or to differences in adaptation to culture conditions. The binding capacity of parasitized cells from subjects with cerebral malaria did not differ from that of IRBCs from subjects with less serious clinical manifestations. |
| first_indexed | 2024-03-06T20:41:35Z |
| format | Journal article |
| id | oxford-uuid:34796849-dcee-4124-8913-7397b2090d7a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-06T20:41:35Z |
| publishDate | 1988 |
| record_format | dspace |
| spelling | oxford-uuid:34796849-dcee-4124-8913-7397b2090d7a2022-03-26T13:26:05ZPlasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:34796849-dcee-4124-8913-7397b2090d7aEnglishSymplectic Elements at Oxford1988Marsh, KMarsh, VBrown, JWhittle, HGreenwood, BAn in vitro model of Plasmodium falciparum-infected red blood cell sequestration which uses C32 amelanotic melanoma cells as targets has been used to examine the binding capacity of infected red blood cells from subjects with naturally acquired P. falciparum infections of varying severity. The binding of infected red blood cells (IRBCs) to melanoma cells was specific to cells containing mature parasites. Variations in target cell density and in conditions of growth had significant effects on binding. Binding was pH dependent, being maximum at a pH of 6.9. Using standardized conditions the binding capacity of individual isolates of P. falciparum could be measured with a high degree of reproducibility. Binding capacity of IRBCs from 51 subjects between the ages of 6 months and 15 years varied between 12 and 1254 IRBCs per 100 melanoma cells when RBC suspensions at a 1% parasitemia and 4% hematocrit were used. Variation in binding was not related to the level of peripheral parasitemia of the isolate or to differences in adaptation to culture conditions. The binding capacity of parasitized cells from subjects with cerebral malaria did not differ from that of IRBCs from subjects with less serious clinical manifestations. |
| spellingShingle | Marsh, K Marsh, V Brown, J Whittle, H Greenwood, B Plasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration. |
| title | Plasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration. |
| title_full | Plasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration. |
| title_fullStr | Plasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration. |
| title_full_unstemmed | Plasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration. |
| title_short | Plasmodium falciparum: the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration. |
| title_sort | plasmodium falciparum the behavior of clinical isolates in an in vitro model of infected red blood cell sequestration |
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